Preparation method of newcastle diseases, infectious bursal disease bigeminy high immunity-yolk-antibody lyophilized powder

A technology of yolk antibody and bursal disease, applied in the field of disease immunity, can solve the problems of high mortality and serious damage to the chicken industry

Inactive Publication Date: 2012-10-24
ZHENGZHOU HOUYI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High mortality rate, serious damage to chicken industry

Method used

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  • Preparation method of newcastle diseases, infectious bursal disease bigeminy high immunity-yolk-antibody lyophilized powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation method of chicken Newcastle disease, infectious bursal disease double-linked hyperimmune egg yolk antibody freeze-dried powder of the present invention, its steps are as follows:

[0029] 1) Select a standard strain to make a vaccine, and immunize selected healthy chickens at intervals of 5 days. Newcastle disease antibody and bursa antibody are carried out alternately. After the second immunization, antibody titer detection begins. Newcastle disease antibody When the titer reaches 1:256 and the bursa antibody titer reaches 1:512, start to collect hyperimmune eggs;

[0030] 2) The collected high-free eggs are cleaned, disinfected, and dried for later use;

[0031] 3) Separate the high-free egg yolk, add an equal volume of sterile water, stir and mix until the color turns white, and get a fully mixed egg yolk solution; stir and mix until it turns white, so that the egg yolk can be broken completely and the antibody release is more effective completely; ...

Embodiment 2

[0056] The preparation method of chicken Newcastle disease, infectious bursal disease double-linked hyperimmune egg yolk antibody freeze-dried powder of the present invention, its steps are as follows:

[0057] 1) Select standard virus strains to prepare vaccines for future use;

[0058] 2) The first immunization of these two vaccines is 0.5mL, and the interval between each immunization is 5 days, and the amount of subsequent immunizations is doubled, and the two vaccines are used to immunize healthy chickens alternately;

[0059] 3) Collect the high-free eggs, sterilize the high-free eggs, dry them, separate the egg yolk, break it up, add an equal volume of sterile water, stir and mix until the color turns white, and obtain the egg yolk solution;

[0060] 4) Put the above-mentioned egg yolk liquid into a 65°C water bath and heat for 30 minutes, stirring intermittently during this period to make it evenly heated.

[0061] 5) After heating, add 6 times pre-cooled hydrochloric ...

Embodiment 3

[0071] The preparation method of chicken Newcastle disease, infectious bursal disease double-linked hyperimmune egg yolk antibody freeze-dried powder of the present invention, its steps are as follows:

[0072] 1) Select standard virus strains to prepare vaccines for future use;

[0073] 2) The first immunization of these two vaccines is 0.5mL, and the interval between each immunization is 5 days, and the amount of subsequent immunizations is doubled, and the two vaccines are used to immunize healthy chickens alternately;

[0074] 3) Collect the high-free eggs, sterilize the high-free eggs, dry them, separate the egg yolk, break it up, add an equal volume of sterile water, stir and mix until the color turns white, and obtain the egg yolk solution;

[0075] 4) Put the above-mentioned egg yolk liquid into a 63°C water bath and heat for 30 minutes, stirring intermittently during this period to make it evenly heated.

[0076] 5) After heating, add 6 times pre-cooled hydrochloric ...

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Abstract

The invention relates to a preparation method of newcastle diseases, infectious bursal disease bigeminy high immunity-yolk-antibody lyophilized powder. The bigeminy yolk-antibody lyophilized powder is prepared by preparing vaccines by preparing strains with strong immunity, preparing high immunity eggs, conducting disinfection, acidizing, octanoic acid treatment, refined exaction purification treatment, preparation and freeze-drying. The newcastle diseases, infectious bursal disease bigeminy high immunity-yolk-antibody lyophilized powder prepared by using the method is a preparation capable of simultaneously preparing the newcastle diseases and chicken infectious bursal diseases, clinically reduces manipulation strength and reduces raise cost. A newcastle diseases, infectious bursal disease bigeminy high immunity-yolk-antibody lyophilized preparation is convenient to transport and store, is long in saving time and can keep high valence of antibody.

Description

technical field [0001] The invention relates to a preparation method of a chicken Newcastle disease and infectious bursal disease double-linked hyperimmune yolk antibody freeze-dried powder, which belongs to the technical field of disease immunity. Background technique [0002] Chicken Newcastle disease and chicken infectious bursal disease are common diseases in the chicken industry, which are prone to large-scale outbreaks and cause serious economic losses. Among them, Newcastle disease (NewCastledisease) is a highly contagious infectious disease caused by paramyxovirus. Also known as Asian chicken plague or pseudo chicken plague. Often presents symptoms of acute septicemia Newcastle disease chicken Newcastle disease. The main features are dyspnea, loose stools, nervous disturbances, and mucosal and serosal hemorrhages. The mortality rate is high, and the poultry industry is seriously damaged. It was first discovered in Indonesia in 1926, and soon found in Newtown, Eng...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61K9/19A61P31/14
Inventor 吴红云徐进哈斯通拉嘎郭俊清
Owner ZHENGZHOU HOUYI PHARMA
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