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Treatment and prevention of cancers associated with intracellular oncoproteins through antibody therapy or vaccination

A cancer and metastatic cancer technology, applied in the field of cell biology and medicine, can solve unused problems and achieve the effect of reducing the formation of breast tumors

Active Publication Date: 2016-08-24
AGENCY FOR SCI TECH & RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibodies have so far not been used to target intracellular antigens or cancer markers due to the inability of antibodies to cross cell membranes making access to antigens

Method used

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  • Treatment and prevention of cancers associated with intracellular oncoproteins through antibody therapy or vaccination
  • Treatment and prevention of cancers associated with intracellular oncoproteins through antibody therapy or vaccination
  • Treatment and prevention of cancers associated with intracellular oncoproteins through antibody therapy or vaccination

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0274] Production of anti-intracellular oncoprotein antibodies

[0275] Anti-intracellular oncoprotein antibodies can be produced by recombinant DNA methods or synthetic peptide chemistry well known to those of ordinary skill in the art.

[0276] For example, anti-intracellular oncoprotein antibodies can be synthesized by techniques well known in the art, as exemplified by "Solid Phase Peptide Synthesis: A Practical Approach" E. Atherton and R.C. Sheppard, IRL Press, Oxford England. Similarly, multiple fragments can be synthesized and then joined together to form larger fragments. These synthetic peptide fragments can also be prepared by amino acid substitutions at specific positions for testing in vitro and in vivo activity.

[0277] Anti-intracellular oncoprotein antibodies can be synthesized in standard microchemical equipment and checked for purity using HPLC and mass spectrometry. Methods of peptide synthesis, HPLC purification and mass spectrometry are known to those s...

Embodiment

[0378] Our results suggest that when a tumor-specific antigen is challenged, host immunity can be stimulated to produce endogenous antibodies against the specific antigen, leading to tumor suppression. The concept of a "cancer vaccine" has been a promising but challenging opportunity 21 . Importantly, we found that antigen-induced antibody therapy achieved similar antitumor efficacy compared with exogenously delivered antibodies. In addition to being more economical, we believe the former approach is more useful than the latter due to our inherent enormous potential adaptability to self-generate high titers of antigen-induced anti-tumor antibodies. Although vaccination with "oncoproteins" does not seem like a realistic idea, we firmly believe that this approach is worth investigating in the future. An "oncoprotein" should be properly located in its natural subcellular location, properly associated with its neighboring partners to maintain oncogenic roles in cancer cell signa...

Embodiment E1

[0380] Example Part E1 (Examples 1 to 18). Proof of Concept Using Antibody Therapy or Vaccination to Target Intracellular Oncoproteins

[0381] Part E1 includes Examples 1 to 18. Embodiment 1 is the introduction to E1 part, and embodiment 2 to 10 is the material and method for E1 part, and embodiment 11 to 16 is the result of E1 part, and embodiment 17 is the discussion of E1 part, and embodiment 18 is E1 Part of the references.

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Abstract

We provide the application of intracellular antibody therapy to target intracellular oncoproteins or proteins to prevent cancer metastasis. We also provide the use of intracellular vaccine therapy comprising oncoprotein proteins for vaccination to stimulate the host to produce its own antibodies to prevent tumor formation. Antibodies can include chimeric antibodies.

Description

technical field [0001] The present invention relates to the fields of cell biology, molecular biology and biochemistry. The invention also relates to the medical field. In particular, the invention relates to the prevention and treatment of diseases, especially cancer, and compositions for such use. Background technique [0002] Cancer has been the subject of serious research over the past two decades. However, the underlying causes of cancer metastasis are poorly understood, and prevention of most types of cancer remains limited. Effective ways to prevent cancer metastasis are urgently needed. [0003] Antibody-based therapies have proven effective in the treatment of cancer; however, this approach is generally limited to extracellular or secreted proteins expressed by cancer cells 1，2 . [0004] Thus, the literature identifies many potential cancer or tumor markers and cancer antigens, and antibody therapies have been developed from some of them. [0005] For example...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/02A61P35/00A61K39/395A61P37/04
CPCA61K39/0011A61K2039/505A61K2039/55566C07K16/32C07K16/40C07K2317/24C07K2317/73C07K2317/77A61P35/00A61P37/04Y02A50/30A61K2039/80
Inventor 曾琦
Owner AGENCY FOR SCI TECH & RES
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