Protein imprint automatic interpretation method and interpretation device thereof

Abstract

The invention discloses a protein imprint automatic interpretation method and an interpretation device thereof. A detected film strip sample is directly shot from top to bottom through a camera based on the machine vision technology, so that a film strip image can be obtained instantly; the requirement on the position accuracy of the film strip is not high, the risk of bio-contamination is avoided, the film strip position can be automatically judged through a virtual recognition frame, and the detection result is automatically recognized as per the type of the film strip and stored in a data base, so that work intensity of inspecting staff is greatly reduced, the working efficiency is improved, and the personal error and difference, particularly the bio-contamination risk during the experiment can be reduced; and direct contact among the film strip, the interpretation equipment and the experiment staff is avoided, and the requirements and trend of biosafety in a laboratory are satisfied.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and relates to an automatic interpretation method of western blot and a corresponding interpretation device. Background technique [0002] With the development of clinical detection technology, automatic Western blot detection is gradually becoming popular. Compared with manual detection, automatic detection can detect a large number of samples at one time. However, the judgment of the test results basically depends on the judgment of the human eye. Due to the strong subjectivity of manual judgment, it is easy to form individual differences. [0003] At present, there is also a system that realizes automatic judgment by scanning with a scanner, which can eliminate the errors of traditional manual judgment, and its judgment results are consistent and quantitative. However, the disadvantage of the existing scanner interpretation system is that it takes more than 1 minute for the scann...

AI Technical Summary

Problems solved by technology

However, the disadvantage of the existing scanner interpretation system is that it takes more than 1 minute for the scanner to scan a sample image of a Western blot membrane strip, and the preparation work before scanning takes a long time, and each membrane strip sample must be accurately pasted on the template. , as long as there is a slight deviation in the pasting position, it will affect the scanning results, the operation is complicated and inefficient

At the same time, during the pasting and scanning process, since the membrane sample contaminated by unknown serum needs to be taken and pasted by hand, and it also needs to be in direct contact with the glass of the scanner, the experimental specimens and interpretation equipment cannot be effectively cleaned and disinfected, and the safety of biological contamination The hidden danger is very big

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