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Human endostatin gene transformed bifidobacterium double-layer microcapsule embedding technology

A technology of endostatin and bifidobacteria, which is applied in the direction of being fixed on/in organic carriers, etc., can solve the problems of unsatisfactory preservation of live bacteria in liquid products, few varieties, and low survival rate of live bacteria.

Inactive Publication Date: 2012-11-07
湖南科尔生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Probiotics have "three fears" (fear of water, oxygen, and gastric acid). In 2003, through the investigation of 55 kinds of probiotic products in the European market: only 13% of the products were qualified, and the current microecological preparation industry in my country is even more There are obvious quality problems: poor strains, few varieties, low content of live bacteria, low survival rate of live bacteria, short survival period of live bacteria, low preparation level
The preservation of live bacteria in liquid products is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Put the homogenized bacteria solution and enteric gel coating solution into the fluidized spray at a ratio of 1:1. The process conditions are: liquid inlet speed 4mL / min, inlet temperature 20.5°C, outlet temperature 20.5°C, nozzle atomization pressure 0.3MPa, Dry air flow: 350m3 / h. The coating rate is 95%. Observed under a low-magnification microscope, it can be seen that the microcapsules are small spherical, with a diameter of 10-30 μm, live bacteria gather at the core of the microcapsules, and each microcapsule contains 90-180 live bacteria cells.

[0016]

Embodiment 2

[0018] Put the homogenized bacteria solution and enteric gel coating solution into the fluidized spray at a ratio of 1:1. The process conditions are: liquid inlet speed 5mL / min, inlet temperature 30.5°C, outlet temperature 25.5°C, nozzle atomization pressure 0.4MPa, Dry air flow: 400m3 / h. The coating rate is 94%. Observed under a low-magnification microscope, it can be seen that the microcapsules are small spherical, with a diameter of 10-30 μm, live bacteria gather at the core of the microcapsules, and each microcapsule contains 110-190 live bacteria cells.

[0019]

Embodiment 3

[0021] The homogenized bacterial liquid and enteric gel coating liquid are put into the fluidized spray at a ratio of 1:1. The process conditions are: liquid inlet speed 6mL / min, inlet temperature 34.5°C, outlet temperature 29.5°C, nozzle atomization pressure 0.5MPa, Dry air flow: 450m3 / h. The coating rate is 98%. Observed under a low-magnification microscope, it can be seen that the microcapsules are small spherical, with a diameter of 10-30 μm, live bacteria gather at the core of the microcapsules, and each microcapsule contains 100-200 live bacteria cells.

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Abstract

The invention discloses a human endostatin gene transformed bifidobacterium double-layer microcapsule embedding technology and solves the problem that a transgene bifidobacterium engineering strain is not easy to preserve and is not resistant to gastric acid, wherein the problem hasn't been broken through for a long time. The technology comprises steps of: using milk protein and the like as a first layer for cladding, using Arabic gum, cello-oligosaccharide and the like as a second layer for embedding, and adopting a fluidization spraying method to transform human endostatin gene bifidobacterium to prepare a microcapsual powder. Through successful development of the project provided by the invention, the technology has very ideal effects for preservation, preparation, gastric acid resistance and colonization of the transgene bifidobacterium engineering strain and paves the way for the development of dosage forms of micro-ecological preparations.

Description

technical field [0001] The invention relates to a double-layer microcapsule embedding technology of bifidobacterium transfected with human endostatin gene. It includes double-layer embedding of human endostatin gene-transferred bifidobacterium cells with milk protein and special colloid. Belongs to the field of biotechnology. Background technique [0002] The research on the beneficial functions of probiotics has a history of nearly a hundred years, and in recent years, probiotic products (microecological preparations) on the market have also emerged in an endless stream, but these products must have sufficient probiotics to truly realize their functions. activity, and can enter the human body to colonize in order to have an effect on the human body. Probiotics have "three fears" (fear of water, oxygen, and gastric acid). In 2003, through the investigation of 55 kinds of probiotic products in the European market: only 13% of the products had qualified activity, while the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/02
Inventor 肖宏苏小平
Owner 湖南科尔生物技术有限公司
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