Rat primary auditory cortex microelectrode array implantation fixation operation method

Abstract

The invention belongs to the research field of electro-neurophysiology, and particularly relates to a rat primary auditory cortex microelectrode array implantation fixation operation method for auditory sense cognitive research. The method comprises the following steps of: fixing a wire electrode part and an electrode connector part of a microelectrode array in a double ectopia way, i.e. fixing the wire electrode on an auditory cortex temporal bone through an electrode landing device, and then buckling the wire electrode to fix the electrode connector onto a parietal bone so as to complete implantation fixation of the auditory cortex microelectrode array. According to the method, the operation is simple, electrodes can be carried stably, the reliability of signal acquisition is high, the requirement of an auditory sense cognitive research under a freetime state of a rat is met, and the experimental animal cost of correlational researches is reduced.

Description

technical field [0001] The invention belongs to the field of neuroelectrophysiology research, and in particular relates to an operation method for implanting and fixing microelectrode arrays in the primary auditory cortex of rats, so as to meet the needs of collecting neuron activities in the rats' auditory cortex in a freely moving state. Background technique [0002] Multi-channel in vivo recording technology can record group neuron activities in local brain regions when animals are awake and freely moving. It is a powerful tool for analyzing the coding mechanism of group neurons at the network level, and is widely used in multiple animal experiments. brain area. However, the multi-channel in vivo recording studies of the auditory cortex are mostly carried out on large experimental animals such as marmosets. For small rodents such as rats, which are commonly used experimental animals, only under anesthesia and passive listening (unanesthetized). Passively listening) group...

AI Technical Summary

Problems solved by technology

When studying the cognitive encoding of auditory sensory information by the high-level central nervous system, it is often necessary to investigate the dynamic changes in the response of central-related brain regions to acoustic stimuli. It is very important to understand the mechanism of central sensory cognition, but there is no report on the neuron activity of rat auditory cortex in the state of behavior

The reason is that the primary auditory cortex of rats and other small experimental animals is located on the temporal side of the brain. If the implanted microelectrode array is directly fixed on the temporal skull outside the auditory cortex, the fixed electrode device will cause the normal balance state of the animal's head to change. changes, which will affect the animal's free movement

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