Biological modification process of traditional Chinese medicine pinellin
A technology of biological modification and pinellia, which is applied in animal husbandry and other fields, can solve the problems of wasting resources and reducing drug efficacy, and achieve the effect of reducing toxicity and expanding the scope of medicinal use
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specific Embodiment approach 1
[0024] Specific embodiment one: the biomodification process of the traditional Chinese medicine pinellia protein of the present embodiment comprises the following steps:
[0025] (1) 1000g of traditional Chinese medicine pinellia is puffed and pulverized with a room temperature puffing device (ZL200520108339.X), and sieved with a sieve with an aperture of 0.1-1.0mm. The powdered pinellia under the sieve is collected and heated in a microwave oven at 915~2450MHz, 500-1000W Sterilize for 1-10 minutes;
[0026] (2) Add 0.5-2L of deionized water to 10.0g of sterilized pinellia and stir to form pinellia paste, add 20-50g of wheat bran, 0.1-0.5g of K 2 HPO 4 and 0.5~2g of peptone, stir evenly, keep the temperature at 25~38°C, time 48-240h, give birth to eggs, use a 0.1mm-2mm sieve to sieve the eggs for later use;
[0027] (3) Take 100g pinellia powder, 2-5g glucose, 0.2-1.0g peptone, 0.2-1.0g KH 2 PO 4 , 0.01-0.05gMgSO 4 , add 0.5-1.0L deionized water, heat at 80-100°C for 10-3...
specific Embodiment approach 2
[0034] Specific embodiment two: the biomodification process of the traditional Chinese medicine pinellia protein of the present embodiment comprises the following steps:
[0035] (1) 1000g of dried pinellia is crushed with a pulverizer at 250-1250r / min to 0.05-0.5mm, and sieved with a 0.05-0.2mm aperture sieve to collect the powdery material;
[0036] (2) Add 5-20L pure water to the powdery material and stir to form a paste;
[0037] (3) The paste is sterilized at 80-120°C for 10-30 minutes, and cooled to 25-30°C for later use;
[0038] (4) Take 100g rice bran, 20-50g paste, 5-10g peptone, 5-10g KH 2 PO 4 Mix evenly, spray 10-15mL of pure water, keep the temperature at 25-55°C for 48-120 hours to generate eggs, and use a sieve with an aperture of 0.1-2.0mm to screen out the eggs for later use;
[0039] (5) Take 200-300g pinellia paste, 5-10g yeast extract powder, 2-5gNaCl, 1-2mL ammonia water, add 0.5-1.0L pure water, mix evenly, and divide into 20-25 triangular flasks , e...
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