Biological fluorescence microscopic detection instrument

Abstract

The invention provides a biological fluorescence microscopic detection instrument which comprises an excitation light illumination unit, excitation light, a fluorescence isolation unit, a reflecting unit, a microimaging unit, a fluorescence detection unit, a precision displacement platform and a control unit. An illumination pinhole is arranged between a first beam expander and a second beam expander of the excitation light illumination unit and located at a focal point of the first beam expander, an imaging detection pinhole is arranged between an imaging lens and a photomultiplier of the fluorescence detection unit and located at a focal point of the imaging lens, and therefore, the transverse resolution of the instrument is effectively improved; meanwhile, an annular beam shaping assembly is arranged between a tube lens and a microobjective of the microimaging unit, which enables the axial resolution of the biological fluorescence microscopic detection instrument to be improved.

Description

Technical field [0001] The invention relates to the field of design and manufacture of microscopic detection instruments, in particular to a biological fluorescence microscopic detection instrument. Background technique [0002] Total internal reflection microscopy imaging technology and confocal microscopy imaging technology have their own advantages and disadvantages: total internal reflection microscopy imaging has high axial resolution, but its lateral resolution is low; confocal microscopy imaging has high resolution The lateral resolution, but its axial resolution is poor. How to simultaneously utilize the high axial resolution of total internal reflection microscopy and the high lateral resolution of confocal microscopy in a microscope is particularly important. [0003] The invention patent application CN201080024155.9 proposes an image processing device, program and microscope that simply and accurately overlap the total internal reflection fluorescence image and the conf...

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Problems solved by technology

[0002] Total internal reflection microscopy and confocal microscopy have their own advantages and disadvantages: total internal reflection microscopy has high axial resolution, but its lateral resolution is low; confocal microscopy has high lateral resolution, but poor axial resolution

Although this method generates a superposed image of the total internal reflection fluorescence image and the confocal image, because the two microscopes work successively, it is physically impossible to obtain a three-dimensional high-resolution image at the same time; in addition, the invention needs to operate two microscopes successively to shoot images, and to overlap the images, the structure of the microscope is more complicated, and the composition of the control system is also more complicated

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