Application of Wujin tablets for preparing medicines for suppressing PC-3 cell proliferation

A PC-3, cell proliferation technology, used in drug combinations, antitumor drugs, pharmaceutical formulations, etc., can solve problems such as no prostate cancer cell proliferation

Inactive Publication Date: 2012-12-19
NANJING ZHENGLIANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Wujin Tablet is the exclusive product of Yunnan Yongzitang Pharmaceutical Co., Ltd., and there is no literature report on its inhibition of prostate cancer (PC-3) cell proliferation

Method used

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  • Application of Wujin tablets for preparing medicines for suppressing PC-3 cell proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] Experimental Research Data of Wujin Tablets Inhibiting PC-3 Cell Proliferation

[0009] 1 Experimental materials

[0010] 1.1 Experimental cell lines

[0011] Prostate cancer (PC-3), from the laboratory cell bank of our company, was routinely cultured in DMEM+10%FBS.

[0012] 1.2 Experimental drugs

[0013] Research drug: Wujin Tablets, Yunnan Yongzitang Pharmaceutical Co., Ltd., batch number: 20110423

[0014] Liquid stock solution: Weigh 100mg of Wujin Tablets and pulverize them, dissolve them in 5ml of absolute ethanol, filter through a 0.2μm filter, aliquot into 500μl doff tubes, store at -20°C, and filter absolute ethanol through a 0.2μm filter for use in the control group .

[0015] 1.3 Experimental reagents

[0016] DMEM (GIBCO Cat.No.12100-061 Lot.No.758137); Fetal Bovine Serum (Zhejiang Tianhang Biotechnology Co., Ltd. Lot.No.100419); NaHCO3 (Shanghai Jiuyi Chemical Reagent Co., Ltd. Cat.No.11810 -033Lot.No.1088387); Trypsin (AMRESCO lot number: 2010 / 04);...

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Abstract

The invention discloses an application of Wujin tablets for preparing medicines for suppressing PC-3 cell proliferation. The formula of the Wujin tablets comprises the components of pollen typhae, plant soot, leonurus, prepared rehmannia root, charred folium artemisiae argyi, rhizoma sparganii, rhizoma corydalis, rhizoma cyperi, white paeony root, fructus psoraleae, fructus evodiae, rhizoma chuanxiong, curcuma zedoary, elecampane, angelica sinensis and fennel oil. The Wujin tablets have the effects of regulating menstruation and removing stasis and are used for qi depression hysteresis, chest and hypochondrium pain, postpartum blood stasis, lower abdomen pain, dysphoria in chestpalms-soles and emaciation with sallow complexion, the specification is 0.6g per tablet, the tablets are taken orally, four tablets are taken once in the mode of two times per day. The tablets can suppress the PC-3 cell proliferation.

Description

technical field [0001] The invention relates to a new application of a Chinese patent medicine, in particular to the application of Wujin tablets in the preparation of medicines for inhibiting PC-3 cell proliferation. Background technique [0002] Prostate cancer is a malignant tumor that occurs in male prostate tissue and is the result of abnormal and disordered growth of prostate acinar cells. Prostate cancer incidence varies markedly by geographic and ethnic origin. In developed countries and regions such as Europe and the United States, it is the most common malignant tumor in men, and its mortality rate ranks second among various cancers; in Asia, its incidence rate is lower than that in Western countries, but it has shown a rapid upward trend in recent years. [0003] Wujin Tablet is the exclusive product of Yunnan Yongzitang Pharmaceutical Co., Ltd. There is no literature report on its inhibition of prostate cancer (PC-3) cell proliferation. Contents of the inventi...

Claims

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Application Information

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IPC IPC(8): A61K36/9066A61P35/00
Inventor 不公告发明人
Owner NANJING ZHENGLIANG MEDICAL TECH
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