Application of Wujin tablets for preparing medicines for suppressing PC-3 cell proliferation
A PC-3, cell proliferation technology, used in drug combinations, antitumor drugs, pharmaceutical formulations, etc., can solve problems such as no prostate cancer cell proliferation
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[0008] Experimental Research Data of Wujin Tablets Inhibiting PC-3 Cell Proliferation
[0009] 1 Experimental materials
[0010] 1.1 Experimental cell lines
[0011] Prostate cancer (PC-3), from the laboratory cell bank of our company, was routinely cultured in DMEM+10%FBS.
[0012] 1.2 Experimental drugs
[0013] Research drug: Wujin Tablets, Yunnan Yongzitang Pharmaceutical Co., Ltd., batch number: 20110423
[0014] Liquid stock solution: Weigh 100mg of Wujin Tablets and pulverize them, dissolve them in 5ml of absolute ethanol, filter through a 0.2μm filter, aliquot into 500μl doff tubes, store at -20°C, and filter absolute ethanol through a 0.2μm filter for use in the control group .
[0015] 1.3 Experimental reagents
[0016] DMEM (GIBCO Cat.No.12100-061 Lot.No.758137); Fetal Bovine Serum (Zhejiang Tianhang Biotechnology Co., Ltd. Lot.No.100419); NaHCO3 (Shanghai Jiuyi Chemical Reagent Co., Ltd. Cat.No.11810 -033Lot.No.1088387); Trypsin (AMRESCO lot number: 2010 / 04);...
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