Mixed sugar for erythromycin fermentation and supplement and application method

An application method and technology of erythromycin, which is applied in the field of mixed sugar for erythromycin fermentation feed, can solve the problems of easy deviation from erythromycin, excessive accumulation, low pH, etc., so as to accelerate the synthesis rate, increase the fermentation unit, and promote the synthetic effect

Active Publication Date: 2014-12-10
NINGXIA QIYUAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of glucose-based feeding are: (1) The pH of the glucose solution itself is low (4.6-5.2), and the pH is lower during the fermentation and metabolism of erythromycin, which easily deviates from the optimal pH value for erythromycin synthesis ( 6.5~7.5)
(2) Glucose is supplemented during the fermentation process of erythromycin, and the metabolic rate of glucose is relatively fast, which does not match the growth rate and product synthesis rate of erythromycin-producing bacteria. The synthesis of erythromycin precursor substances slows down the synthesis rate of erythromycin products, that is, reduces the fermentation unit of erythromycin in the tank

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Add 0.2% medium-temperature amylase to starch milk with a mass concentration of 35%, the pH is 6.0-8.0, the heating temperature is between 75-80°C, hydrolyzed to a DE value of 20%, the temperature is raised to 100°C, and then lowered to At room temperature, the pH is between 6.0 and 8.0. According to the fermentation requirements, feed sugar with a concentration of 30% is prepared and sterilized. The sterilization temperature is controlled at 118-121° C., the pressure is 0.1 MPa, and the sterilization is performed for 20-30 minutes. After erythromycin was fermented to 22 hours, the sterilized mixed sugars were added by fed-batch method. After metabolism, the process pH was 6.5-7.5, and the process reducing sugars were controlled at 0.2-3.0%. 166 hours into the can, the unit of the can is 8100u / ml, which is 16.55% higher than that of the comparative experiment.

Embodiment 2

[0019] The starch milk with a mass concentration of 25% is added with 0.4% of medium temperature amylase, the pH is 6.0-8.0, the heating temperature is between 75-80°C, hydrolyzed to a DE value of 20%, the temperature is raised to 100°C, and then lowered to At room temperature, the pH is between 6.0 and 8.0. According to the fermentation requirements, feed sugar with a concentration of 20% is prepared and sterilized. The sterilizing temperature is controlled at 118-121° C., the pressure is 0.1 MPa, and the sterilizing is performed for 20-30 minutes. After erythromycin was fermented for 19 hours, the sterilized mixed sugars were added in fed-batch mode. After metabolism, the process pH was 6.5-7.5, and the process reducing sugars were controlled at 0.2-3.0%. 162 hours into the tank, the tank unit is 7850u / ml, which is 12.95% higher than the comparison experiment.

Embodiment 3

[0021] Add 0.5% medium-temperature amylase to starch milk with a mass concentration of 30%, the pH is 6.0-8.0, the heating temperature is between 75-80°C, hydrolyzed to a DE value of 20%, the temperature is raised to 100°C, and then lowered to At room temperature, the pH is between 6.0 and 8.0. According to the fermentation requirements, feed sugar with a concentration of 25% is prepared and sterilized. The sterilization temperature is controlled at 118-121° C., the pressure is 0.1 MPa, and the sterilization is performed for 20-30 minutes. After erythromycin is fermented for 20 hours, the sterilized mixed sugar is added, and the process pH is 6.5-7.5 after metabolism, and the process reducing sugar is controlled at 0.2-3.0%. 172 hours into the can, the unit of the can is 8450u / ml, which is 21.58% higher than that of the comparative experiment.

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Abstract

The invention relates to mixed sugar for erythromycin fermentation and supplement and an application method of the mixed sugar. The method comprises the following steps: hydrolyzing starch milk of which the mass concentration is 25-35% by medium-temperature amylase under the conditions that the pH is 6.0-8.0 and the temperature is 75-80 DEG C until the value of glucose is 10-30%; continually heating the starch milk to 100 DEG C, inactivating the medium-temperature amylase, then cooling to room temperature, and feeding the amylase into a sterilization tank to be sterilized according to the mass concentration of 20-30% to obtain the mixed sugar of which the pH is 6.0-8.0 and the concentration is 20-30%. According to the mixed sugar for the erythromycin fermentation and supplement and the application method of the mixed sugar, the starch milk is processed into the mixed sugar containing monosaccharide, disaccharide and polysaccharide by the amylase; the tolerance on high-temperature and high-pressure sterilization is good because of containing the starch milk; other by-products are not easily generated in a sterilization process, and the pH, metabolism pH and the metabolism rate are suitable for growth and pigment-producing capacity of an erythromycin-producing strain, therefore, synthesis of erythromycin precursor substances can be facilitated; the synthesis rate of an erythromycin product is accelerated, and the fermentation unit of the erythromycin is improved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, in particular to a mixed sugar for erythromycin fermentation feed and an application method thereof. Background technique [0002] Erythromycin fermentation uses an actinomycete (Streptomyces rubrum) as the producing bacteria. After rejuvenation, separation, and spore culture, slant spores are prepared for use. Then, the slant spores are inserted into the seed tank for seed expansion cultivation, and finally fermented. Compound culture medium is a process of maximizing the production of erythromycin by controlling parameters such as culture temperature, PH, and matrix concentration. [0003] In the current fermentation process of erythromycin fermentation, the feeding material in the fermentation process is mainly to add simple sugar, namely glucose. The main disadvantages of glucose-based feeding are: (1) The pH of the glucose solution itself is low (4.6-5.2), and the pH is low...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/62C12R1/465
Inventor 李学兵孙瑞君朱娜张志
Owner NINGXIA QIYUAN PHARMA
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