Mixed sugar for erythromycin fermentation and supplement and application method

Abstract

The invention relates to mixed sugar for erythromycin fermentation and supplement and an application method of the mixed sugar. The method comprises the following steps: hydrolyzing starch milk of which the mass concentration is 25-35% by medium-temperature amylase under the conditions that the pH is 6.0-8.0 and the temperature is 75-80 DEG C until the value of glucose is 10-30%; continually heating the starch milk to 100 DEG C, inactivating the medium-temperature amylase, then cooling to room temperature, and feeding the amylase into a sterilization tank to be sterilized according to the mass concentration of 20-30% to obtain the mixed sugar of which the pH is 6.0-8.0 and the concentration is 20-30%. According to the mixed sugar for the erythromycin fermentation and supplement and the application method of the mixed sugar, the starch milk is processed into the mixed sugar containing monosaccharide, disaccharide and polysaccharide by the amylase; the tolerance on high-temperature and high-pressure sterilization is good because of containing the starch milk; other by-products are not easily generated in a sterilization process, and the pH, metabolism pH and the metabolism rate are suitable for growth and pigment-producing capacity of an erythromycin-producing strain, therefore, synthesis of erythromycin precursor substances can be facilitated; the synthesis rate of an erythromycin product is accelerated, and the fermentation unit of the erythromycin is improved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, in particular to a mixed sugar for erythromycin fermentation feed and an application method thereof. Background technique [0002] Erythromycin fermentation uses an actinomycete (Streptomyces rubrum) as the producing bacteria. After rejuvenation, separation, and spore culture, slant spores are prepared for use. Then, the slant spores are inserted into the seed tank for seed expansion cultivation, and finally fermented. Compound culture medium is a process of maximizing the production of erythromycin by controlling parameters such as culture temperature, PH, and matrix concentration. [0003] In the current fermentation process of erythromycin fermentation, the feeding material in the fermentation process is mainly to add simple sugar, namely glucose. The main disadvantages of glucose-based feeding are: (1) The pH of the glucose solution itself is low (4.6-5.2), and the pH is low...

AI Technical Summary

Problems solved by technology

The main disadvantages of glucose-based feeding are: (1) The pH of the glucose solution itself is low (4.6-5.2), and the pH is lower during the fermentation and metabolism of erythromycin, which easily deviates from the optimal pH value for erythromycin synthesis ( 6.5~7.5)

(2) Glucose is supplemented during the fermentation process of erythromycin, and the metabolic rate of glucose is relatively fast, which does not match the growth rate and product synthesis rate of erythromycin-producing bacteria. The synthesis of erythromycin precursor substances slows down the synthesis rate of erythromycin products, that is, reduces the fermentation unit of erythromycin in the tank