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Primer pair, kit and detection method for detecting humpback grouper

A technology of primer pair and camelback bass, which is applied in the directions of biochemical equipment and methods, determination/inspection of microorganisms, DNA/RNA fragments, etc., to achieve the effects of high sensitivity, high sensitivity and strong specificity

Inactive Publication Date: 2012-12-26
INSPECTION & QUARANTINE TECH CENT OF XIAMEN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Scholars at home and abroad have done little research on the species DNA identification of humpback perch, and there is no report at home and abroad that can quickly, simply, specifically and sensitively detect the PCR method and kit for humpback perch

Method used

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  • Primer pair, kit and detection method for detecting humpback grouper
  • Primer pair, kit and detection method for detecting humpback grouper
  • Primer pair, kit and detection method for detecting humpback grouper

Examples

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Embodiment 1

[0031] 1. Design of primer pair: According to the COI gene sequence of Humpback Bass (Genbank sequence number JF750765 is SEQ ID No: 3) and other grouper COI sequences, the specific primer pair sequence is as follows:

[0032] SEQ ID No: 1: 5'- gCTTCTCCTTgCTTCTTCTg -3'

[0033] SEQ ID No: 2: 5'-gTACAgggAgAgAAAgAAgTAg-3'.

[0034] The primer sequence was synthesized by TAKARA company.

[0035] 2. Synthesis of primer pairs and preparation of kits:

[0036] Based on the above nucleotide sequence information, a primer pair was synthesized by TAKARA.

[0037] Prepare the above-mentioned synthesized primer pairs into a solution with a concentration of 10 μmol / L as a kit for use.

[0038] The upstream primer Calf described in the following examples is SEQ ID No:1, and the downstream primer Calr is SEQ ID No:2.

[0039] 3. Extraction of sample genomic DNA (prepare sample genomic DNA solution according to QIAGEN DNeasy blood and tissue DNA extraction kit):

[0040] 1) After cutting the muscle tissue...

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Abstract

The invention relates to a primer pair, kit and detection method for detecting a humpback grouper. The sequence of the primer pair is shown as SEQ ID NO:1 and SEQ ID NO:2. The invention also includes a kit containing the primer pair and a detection method using the primer pair and the kit. The detection method provided by the invention is simple, quick, specific and sensitive.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular, the invention relates to a pair of primers, a kit and a detection method for detecting humpback perch. Background technique [0002] humpback bass ( Cromileptes altivelis ), also known as mouse spot, is a valuable economic fish with a large market demand and a particularly expensive price. In recent years, with the improvement of people's living standards and the constant changes in demand, the consumption of rare and precious fish has developed extremely rapidly. Some unscrupulous traders frequently seek huge profits through wrong labels, shoddy and false labels, which damage the interests of consumers and Health has also gradually highlighted issues such as food composition, quality and quality safety. In order to protect the rights and interests of consumers, it is necessary to determine the authenticity and species of fish used in products, and to establish reliable and accurate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 陈双雅陈伟玲王嘉鹤张永祥徐敦明周昱
Owner INSPECTION & QUARANTINE TECH CENT OF XIAMEN ENTRY EXIT INSPECTION & QUARANTINE BUREAU