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Electrostatic coating based non-viral nucleic acid ternary complex system and preparation method thereof

An electrostatic coating, non-viral technology, applied in the field of biomedicine, can solve the problems of poor serum stability, short circulation time, difficult long-term stable expression of genes, etc., to enhance lysosome escape function, protect from degradation, improve trans The effect of transfection efficiency and expression efficiency

Inactive Publication Date: 2013-01-02
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is because the surface of the PbAE / DNA complex has a large number of positive charges, which has poor stability to serum, short circulation time in the body, and difficult long-term stable expression of genes.

Method used

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  • Electrostatic coating based non-viral nucleic acid ternary complex system and preparation method thereof
  • Electrostatic coating based non-viral nucleic acid ternary complex system and preparation method thereof
  • Electrostatic coating based non-viral nucleic acid ternary complex system and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1: the synthetic method of cationic polymer poly-β-amino ester (PbAE)

[0036] 5-amino-1-pentanol:1,4-butanediol diacrylate=1.1:1 (mass ratio). Accurately weigh the appropriate amount of 5-amino-1-pentanol and 1,4-butanediol diacrylate according to the mass ratio, place them in a three-necked bottle, and react in an oil bath at 95°C under the condition of magnetic stirring for 12 hours. Under nitrogen protection, viscous yellow liquid was obtained.

Embodiment 2

[0037] Embodiment 2: the synthetic method of cationic polymer poly-β-amino ester (PbAE)

[0038] 5-amino-1-pentanol:1,4-butanediol diacrylate=1.2:1 (mass ratio). Accurately weigh the appropriate amount of 5-amino-1-pentanol and 1,4-butanediol diacrylate according to the mass ratio, place them in a three-necked bottle, and react in an oil bath at 95°C under the condition of magnetic stirring for 12 hours. Under nitrogen protection, viscous yellow liquid was obtained.

Embodiment 3

[0039] Embodiment 3: the synthetic method of cationic polymer poly-β-amino ester (PbAE)

[0040] 5-amino-1-pentanol:1,4-butanediol diacrylate=1.3:1 (mass ratio). Accurately weigh the appropriate amount of 5-amino-1-pentanol and 1,4-butanediol diacrylate according to the mass ratio, place them in a three-necked bottle, and react in an oil bath at 95°C under the condition of magnetic stirring for 12 hours. Under nitrogen protection, viscous yellow liquid was obtained.

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Abstract

The invention falls into the biomedical field and relates to an electrostatic coating based non-viral nucleic acid ternary complex system (TCS) and the preparation method thereof. The TCS is prepared by autonomous compression of plasmid DNA or siRNA through cation polymer poly(beta-amino ester) to form a binary complex, and physical modification of the binary complex with multi-carboxyl polymer through electrostatic interaction. The invention can effectively compress and pack plasmid DNA or siRNA, enhance the lysosome escape of the TCS, and protect the plasmid from degradation in cell, thereby improving the efficiency of gene transfection and expression.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a new three-component non-viral nucleic acid delivery system based on electrostatic coating and a preparation method thereof. Background technique [0002] The realization of gene therapy has brought hope for the cure of many human diseases such as cancer, cardiovascular and cerebrovascular diseases, innate immune diseases and neurological diseases. Practice has shown that a key obstacle in the clinical application of gene therapy is the lack of a safe and effective gene delivery vector system. Gene delivery systems mainly include viral vectors and non-viral vectors, among which viral gene delivery systems are by far the most mature and widely used gene delivery systems. Although viral vectors have high transfection efficiency, many disadvantages limit the application of viral vectors, such as non-specificity, immunogenicity, and easy degradation by enzymes. For example, in...

Claims

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Application Information

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IPC IPC(8): C12N15/63C08G73/00
Inventor 沙先谊顾吉晋方晓玲
Owner FUDAN UNIV
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