Method for measuring sugar through nanometer gold colorimetry

A technology of nano-gold and sugar measurement, applied in the field of analytical chemistry, can solve the problems of a lot of time, expensive detection equipment, and high cost of organic chromophores

Active Publication Date: 2013-01-02
NANJING UNIV OF TECH
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  • Abstract
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Problems solved by technology

Although the detection limit and sensitivity of this type of detection method are high, there are also great limitations: ...

Method used

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  • Method for measuring sugar through nanometer gold colorimetry
  • Method for measuring sugar through nanometer gold colorimetry
  • Method for measuring sugar through nanometer gold colorimetry

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Embodiment 1

[0027] (1) Preparation of nano gold

[0028] Nanogold was prepared by reducing chloroauric acid with sodium citrate (see figure 1 ). First of all, all glassware needs to be soaked in aqua regia to get rid of the remaining reducing substances in the glass container. Accurately weigh HAuCl 4 4H 2 O 0.0123g in a 250mL three-necked flask, and then add 100mL of water to the three-necked flask. Stir vigorously, heat to boil and reflux. Accurately weigh C 6 h 5 Na 3 o 7 2H 2 O0.2849g in a 25mL volumetric flask. Get a certain amount of sodium citrate solution, add the flask quickly after being heated to 50°C in a water bath. After 15 minutes, the solution turned from colorless to light blue, then to purple and finally to wine red. After continuing to heat for 10 minutes, stop heating, continue stirring for 10 minutes and then cool to room temperature. The size of gold nanoparticles is related to the amount of sodium citrate added.

[0029] (2) Detection of glucose

[003...

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Abstract

The invention belongs to the field of analytical chemistry, and relates to a method for measuring sugar through nanometer gold colorimetry. According to the method of the present invention, a solution system containing phenyl boronic acid or a phenyl boronic acid derivative and nanometer gold is added with a sugar-containing sample, the sugar substance in the sample and the phenyl boronic acid or the phenyl boronic acid derivative are combined to inhibit combination of the phenyl boronic acid or the phenyl boronic acid derivative and the nanometer gold so as to change a color of the solution, and quantitative, semi-quantitative or qualitative detection of the sugar substance in the sample is achieved by detecting or observing the change of the solution color. The method of the present invention has the following characteristics that: steps are simple, cost is low, no special equipment is required, chemical modification is not required to be performed on the nanometer gold, and the method of the present invention is a detection method having broad application prospects in food science, analytical chemistry, medicine and other fields.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to a method for measuring sugar by colorimetric nanometer gold. Background technique [0002] The research on sugar is of great significance in the fields of industrial production and life sciences. For example, in the food industry, by measuring the sugar content of food and raw materials, the food processing process can be monitored; by quickly and accurately measuring the sugar content in sugar-free and low-sugar foods , to ensure the safety of sugar-free food; through the determination of blood sugar content in the human body, people's health status can be understood; the study of sugar chains on the surface of cell membranes can explore the mechanism of cancer, bacterial and viral infections and other diseases. However, due to the complex structure of carbohydrates and limited research methods, the research on carbohydrates has lagged behind the research on proteins and nucleic...

Claims

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Application Information

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IPC IPC(8): G01N21/75G01N21/31
Inventor 黄和江凌李燕萍田丹碧李霜
Owner NANJING UNIV OF TECH
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