Separation and purification method of raffinose
A technology for separation and purification of raffinose, applied in the field of chemical engineering, to achieve the effects of low cost, good stability and long service life
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Embodiment 1
[0035] The degreased cottonseed meal is percolated and extracted with ethanol aqueous solution with a volume fraction of 75% to obtain a raffinose extract, and the gossypol and other pigments in the raffinose extract are removed by activated carbon decolorization, and the decolorized solution is further concentrated to obtain concentrated raffinose liquid. Add water to the raffinose concentrate to make a raw material solution with a solid concentration of 200 mg / mL, use Sephadex G-10 (dextran gel, molecular weight exclusion 700) as a filler, and fully swell it with 20% ethanol aqueous solution , using the wet packing method to pack the gel chromatographic column, the chromatographic column size is Φ1.6cm×33cm, and the chromatographic column volume (BV) is 66.3ml. Take 0.02BV of the raw material solution and load it on the packed gel chromatography column. After the sample loading is completed, flow through the gel chromatography column with a volume fraction of 20% ethanol aqu...
Embodiment 2
[0037] The degreased cottonseed meal is percolated and extracted with ethanol aqueous solution with a volume fraction of 75% to obtain a raffinose extract, and the gossypol and other pigments in the raffinose extract are removed by activated carbon decolorization, and the decolorized solution is further concentrated to obtain concentrated raffinose liquid. Add water to the raffinose concentrate to make a raw material solution with a solid concentration of 500mg / mL, use Sephadex LH-20 (hydroxypropyl dextran gel, exclusion molecular weight 4000-5000) After the ethanol aqueous solution is fully swollen, the gel chromatographic column is packed by the wet packing method. The chromatographic column size is Φ1.6cm×33cm, and the chromatographic column volume (BV) is 66.3ml. Take 0.1BV of the raw material solution and load it on the packed gel chromatography column. After the sample loading is completed, use 30% ethanol aqueous solution to flow through the gel chromatography column at...
Embodiment 3
[0039] The degreased cottonseed meal is percolated and extracted with ethanol aqueous solution with a volume fraction of 75% to obtain a raffinose extract, and the gossypol and other pigments in the raffinose extract are removed by activated carbon decolorization, and the decolorized solution is further concentrated to obtain concentrated raffinose liquid. Add water to the raffinose concentrate to make a raw material solution with a solid content concentration of 800 mg / ml, use Sephadex G-50 (dextran gel, exclusion molecular weight range is 1000-5000) as filler, and pass through ethanol with a volume fraction of 30%. After the aqueous solution is fully swollen, the gel chromatographic column is packed by the wet packing method. The chromatographic column size is Φ1.6cm×33cm, and the chromatographic column volume (BV) is 66.3ml. Take 0.05BV of the raw material solution and load it on the packed gel chromatography column. After the sample loading is completed, flow through the g...
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Abstract
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