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Cell separation method

A separation method and cell technology, applied in the field of cell separation, can solve problems such as difficult to overcome over-segmentation, difficult to match pits correctly, and reduce the amount of calculation.

Inactive Publication Date: 2015-04-22
成都聚蚁科技有限公司
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Problems solved by technology

[0006] In order to overcome the problem that the existing cell image separation method is difficult to correctly match the concave points and overcome the "over-segmentation", a special fusion of OSTU and boundary tracking is proposed, and the precise Euclidean distance is used for the previous distance transformation. And a cell separation method that reduces the amount of calculation by proposing the concepts of distance threshold and area threshold

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Embodiment Construction

[0059] The present invention will be further described in detail below in conjunction with the accompanying drawings.

[0060] The basic process of the inventive method is as figure 1 As shown, it specifically includes the following steps:

[0061] A uses the OSTU method for pre-segmentation;

[0062] a1. According to the gray value of the input image, use the OSTU method to calculate the optimal threshold , the specific process is:

[0063] According to the input grayscale image (as attached figure 2 ), divide it into 1~m levels, use The gray level in the representative image is The number of pixels, then the total number of pixels in the image , and the probability that each gray level exists . If you choose a gray level Divide the image into two groups, where , , then the probability generated by the two groups , and the mean difference within the two groups , They are: , , , ,in , representing the average gray value of the entire imag...

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Abstract

The invention relates to the field of image segmentation, in particular to a cell separation method. The cell separation method comprises the following steps: A, pre-segmenting a cell image by an OSTU method; B, performing hole filling on the pre-segmented image by a boundary tracking method; C, performing watershed segmentation on the image subjected to hole filling; and d, combining 'over-segmentation' regions of the segmented image and re-segmenting 'incomplete segmentation' regions. Compared with the conventional segmentation algorithm synthesizing concave point analysis and boundary detection and the conventional segmentation algorithm based on mathematical morphology, the cell separation method is clear in thinking and easy to implement in codes, so that the cell separation method is easy to implement. Compared with the traditional method, the cell separation method can obviously reduce the over-segmentation conditions.

Description

technical field [0001] The invention relates to the field of image segmentation, in particular to a cell separation method. Background technique [0002] The development of microscope technology has enabled human beings to observe things more finely, opening up the study of the microcosm. Gradually enter into the exploration of cells - the basic unit of life activities. In the study of biological cell morphology and structure, it is found that due to the structure of the cell itself, the observed cell image is often a collection of many cells, so it is inevitable to introduce cell segmentation technology, through which the overlapping cells can be accurately Separation, in order to carry out the identification of the cell shape and the statistics of the number in the next step. [0003] There are two main categories of existing segmentation methods: segmentation algorithms that integrate pit analysis and edge detection, and segmentation algorithms based on mathematical mor...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06T7/00
Inventor 李均利李晓宁袁丁苏菡张莹杨军
Owner 成都聚蚁科技有限公司
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