Kit for fast culture, identification and drug sensitivity test of myeoplasmapneumoniae (Mp) and detection method therefor

Abstract

The invention relates to the technical field of myeoplasmapneumoniae (Mp) detection and discloses a kit for fast culture, identification and a drug sensitivity test of Mp and a detection method therefor. The kit comprises a culture tube, a drug sensitivity identification plate and a container unit containing sterile mineral oil. A base liquid, a growth promoting agent, a growth indicator and an impurity bacterium inhibitor are arranged in the culture tube. The drug sensitivity identification plate comprises an identification zone and a drug sensitivity test zone. Through the impurity bacterium inhibitor adopted by a culture solution formula, a false positive problem caused by bacteria and fungi is solved. Through the growth promoting agent and the growth indicator adopted by the culture solution formula, a problem of overlong detection time is solved. Through the identification zone, a false positive problem caused by other mycoplasmas is solved. Through the drug sensitivity test zone, a fast and accurate drug sensitivity test of Mp is realized. Therefore, the kit can effectively avoid false positive and has a short detection period.

Description

technical field [0001] The invention relates to the technical field of mycoplasma pneumoniae detection, in particular to a kit for rapid cultivation, identification and drug sensitivity of mycoplasma pneumoniae, and a detection method for detecting mycoplasma pneumoniae by using the kit. Background technique [0002] Mycoplasma pneumoniae (myeoplasma neumoniae, Mp) is between bacteria and viruses, facultatively anaerobic, and can live independently. More than 1 / 3. [0003] There are several methods for clinical detection of Mycoplasma pneumoniae (MP) infection: [0004] 1. Detection of Mp antibody in serum: This method is quick and easy, and has a high positive rate. However, Mp antibody is the product of human body stimulated by Mp infection. It can only be detected several weeks after infection, and can last for several months to several years. Therefore, the positive result of Mp antibody is not synchronized with Mp infection; [0005] 2. Simple identification culture:...

AI Technical Summary

Problems solved by technology

The culture solution is a simple identification culture solution with a single antibacterial component; the drug-sensitive microplate is prepared according to the method used to detect mycoplasma of urinary system infection (ureaplasma urealyticum, mycoplasma hominis), and does not contain selective identification (such as methylene blue reduction tolerance) function, it is difficult to avoid false positives caused by bacteria, fungi, and other mycoplasma