Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for screening bovine nuclear transplantation donor cells by using antioxidant

A technique of donor cell and nuclear transplantation, applied in botany equipment and methods, biochemical equipment and methods, animal cells, etc., to reduce the chance of wound infection, improve the success rate, and save time and resources

Inactive Publication Date: 2015-04-29
HUAZHONG AGRI UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of β-mercaptoethanol for the cultivation of bovine donor cells has not yet been discovered.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for screening bovine nuclear transplantation donor cells by using antioxidant
  • Method for screening bovine nuclear transplantation donor cells by using antioxidant
  • Method for screening bovine nuclear transplantation donor cells by using antioxidant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0047] 1. Donor cell culture and nuclear transfer

[0048] 1) Sampling: Scrub the tip of the cow's ear for 4-5cm with 75% alcohol 2 Cut the tissue with ophthalmic scissors, wash the tissue three times with 75% alcohol, and then wash it three times with phosphate buffered saline (PBS) containing double antibody, put it into cell culture medium-1, and obtain the bovine ear tissue sample ;

[0049] 2) Cultivate bovine ear fibroblasts by inoculation method: wash the bovine ear tissue samples three times with 75% alcohol and 1% double-antibody PBS respectively, put them in a glass dish, remove the hair and epidermis, and use ophthalmic scissors to peel off the dermal tissue , washed with PBS containing 1% double antibody and cut to 1mm 3 The left and right small tissue pieces are placed in cell culture medium-1, centrifuged at 800r / min for 3min, and washed repeatedly for 6-8 times; add a small amount of cell culture medium-2, and evenly spread the centrifuged and washed tissue pi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the technical field of animal reproduction and molecular biology operation, specifically to a selection method for donor cells in bovine cell nuclear transplantation, and discloses a method for screening bovine nuclear transplantation donor cells by using an antioxidant. In order to improve donor cell quantity in bovine cell nuclear transplantation efficiency, in vivo sampling is adopted; an inoculation method is adopted to culture cells; the sixth generation cells of cells having a stable passage characteristic and a viability OD value of 0.556-0.662 are treated for 24 h by using beta-mercaptoethanol with a concentration of 100 mumol / L, and the obtained cells are adopted as donor cells; and foundation is established for good reconstructed embryo development.

Description

technical field [0001] The invention relates to the field of animal reproductive physiology and biotechnology. Specifically, it relates to a method for selecting donor cells in bovine somatic cell nuclear transfer. Background technique [0002] The current efficiency of nuclear transfer averages less than 10%. The improvement of the efficiency of somatic cell nuclear transfer depends on the further improvement of each link, and the preparation of donor cells is a key step in nuclear transfer. The source and type of donor cells, the donor nuclei at different developmental stages, as well as the number of generations of donor cells cultured in vitro and the age of individual donor cells, all have great differences in the effect of nuclear transfer. [0003] Some current studies are based on the selection of cell algebra, and the results of the research are still inconclusive (Gao Guolong, research on the factors affecting the preparation of donor and recipient cells and the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/873C12N5/071
Inventor 易建明赵玲玲宋海泉杨利国陶林林
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com