Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Single nucleotide polymorphism for prognosis of hepatocellular carcinoma

A single nucleotide polymorphism, hepatocellular carcinoma technology, applied in the field of single nucleotide polymorphism, can solve the problems of low survival rate and high relapse rate, and achieve the effect of improving poor prognosis

Active Publication Date: 2013-01-30
郑永化
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, it was found that with the strong expression of MTA1, the relapse rate after radical surgical resection was high and the survival rate was low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Single nucleotide polymorphism for prognosis of hepatocellular carcinoma
  • Single nucleotide polymorphism for prognosis of hepatocellular carcinoma
  • Single nucleotide polymorphism for prognosis of hepatocellular carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 SNP selection

[0054] Biallelic SNPs contained within ±2 kb of angiogenesis-related genes (ie, VEGF, HIF1a, IGF2, FGF2 or MTA1) were investigated. For the position of the SNP of the gene, refer to the known gene information (http: / / www.ncbi.nlm.nih.gov / project / SNP) for the 5'-untranslated region, promoter region, exon region and gene Seating area to choose from.

[0055] The IDs of the SNPs under study are as follows:

[0056] rs699947、rs25648、rs3025000、rs3025010、rs3025035、rs3025040、rs10434、rs998584、rs45533131 / rs1957757、rs2301113、rs2057482 / rs2585、rs3802971、rs3213221、rs3741212、rs2239681、rs3741208、rs1004446、rs7924316、rs3842748、rs2070762 / rs308395、rs308428、rs11938826、 rs17472986, rs308442, rs308379, rs308381, rs6534367, rs1048201, rs3747676 / rs4983413 and DL1002505.

[0057] Among the above SNPs, SNPs with a haplotype frequency equal to or higher than 5% were selected, and the haplotype frequencies were analyzed by using PHASE software v2.1. In addition, li...

Embodiment 2

[0058] Embodiment 2 SNP genotype analysis

[0059] 1. SNP genotype analysis

[0060] A primer set capable of amplifying the region containing the SNP of Example 1 and a TaqMan probe containing the SNP region were designed by using the primer express software. For TaqMan probes, individual probes for wild-type and mutant alleles were designed according to the sequence of the SNP.

[0061] Probes are made by adding a fluorochrome to one side of a TaqMan probe and a quencher that suppresses the color of the fluorochrome on the other side. In this case, each fluorescent dye with a different color is added for the wild-type and mutant alleles, respectively.

[0062] The three types of primers disclosed in Table 1 below were mixed together, and then a PCR reaction was performed by using the mixed primers, thereby discriminating single nucleotide pair mismatches according to the exonuclease activity property of Taq polymerase.

[0063] [Table 1]

[0064]

[0065]

[0066] A...

Embodiment 3

[0070] Example 3 patient characteristics

[0071] The experiment was conducted on 506 patients with recurrent HCC at Asan Hospital between 1998 and 2003. The clinical characteristics of the 506 patients are shown in Table 2 below. Following radical surgical resection, patients were followed at a mean interval of 43 months (range, 1-96 months).

[0072] [Table 2]

[0073]

[0074] Survival and HCC recurrence were determined by using medical records from the last day of follow-up. For patients who have not been followed up for 3 months, the evaluation is carried out by visiting the nearby residents of the patient.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a single nucleotide polymorphism (SNP) for the prognosis of hepatocellular carcinoma, wherein the SNP shows a significant correlation with an over-expression of MTA1 which is a useful prognostic factor for prediction of recurrence or poor survival after hepatocellular carcinoma surgery. Therefore, the SNP can be used in developing micro-arrays or diagnosis kits for the prognosis of hepatocellular carcinoma, and in screening drugs for improving prognosis of hepatocellular carcinoma to thereby improve poor prognosis of hepatocellular carcinoma after surgery.

Description

technical field [0001] The present invention relates to single nucleotide polymorphisms (SNPs) for predicting the prognosis of hepatocellular carcinoma, wherein the SNPs exhibit a significant correlation with the overexpression of metastatic tumor antigen 1 (MTA1) , the MTA1 is a useful prognostic factor for predicting recurrence (recurrence) or poor survival (poor survival) after surgery for hepatocellular carcinoma; the present invention also relates to a method for predicting the prognosis of hepatocellular carcinoma by using the SNP A microarray or test kit; and a method of screening for a drug that improves prognosis in hepatocellular carcinoma. Background technique [0002] From the perspective of cancer development and death, hepatocellular carcinoma (HCC) is the third most common and serious cancer among all malignancies in Korea. Hepatocellular carcinoma is one of the most vascular tumors. [0003] The most effective modalities of treatment for HCC are surgical re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11G01N33/15C12N15/12
CPCC07H21/04C12Q1/6886C12Q2600/156C12Q2600/118G01N33/15
Inventor 郑永化朴能和维银实李荣柱金精我李丹毕李世焕李宗殷
Owner 郑永化
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com