A kind of traditional Chinese medicine compound thermosensitive gel embolic agent and its preparation method and application

A technology of sensitive gel embolizing agent and traditional Chinese medicine, applied in the field of antitumor preparations, can solve the problems of poor oral absorption, insoluble in water, low bioavailability, etc., and achieve the effects of good fluidity and good MRI visualization function

Active Publication Date: 2022-07-01
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of these compounds are insoluble in water, have poor oral absorption and low bioavailability

Method used

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  • A kind of traditional Chinese medicine compound thermosensitive gel embolic agent and its preparation method and application
  • A kind of traditional Chinese medicine compound thermosensitive gel embolic agent and its preparation method and application
  • A kind of traditional Chinese medicine compound thermosensitive gel embolic agent and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] (1) Preparation and characterization of superparamagnetic iron oxide nanoparticles: Weigh 0.27g FeCl 3 •6H 2 O, 0.75g sodium acrylate and 0.75g anhydrous sodium acetate were placed in a round-bottomed flask, 10mL of diethylene glycol was added, mechanically stirred for 24h, transferred to an autoclave, reacted at 190°C for 10h, after natural cooling, 10mL of Washing with absolute ethanol, centrifugation, repeated three times, and finally vacuum drying to obtain superparamagnetic iron oxide nanoparticles. The particle size, potential and PDI of the prepared superparamagnetic iron oxide nanoparticles were measured by Malvern particle size analyzer, the morphology of the superparamagnetic iron oxide nanoparticles was observed by scanning electron microscope, the iron content was determined by spectrophotometry, and the magnetic resonance imaging was performed. The imaging properties of superparamagnetic iron oxide nanoparticles were measured by instrument.

[0067] like ...

Embodiment 2

[0109] Inhibit HIF-1 α The effect of the expressed traditional Chinese medicine components on HepG2 cells in hypoxic state:

[0110] with CoCl 2 (200μM, 24h) induced hypoxia in HepG2 cells as a cell model, Western blot method was used to compare the effect of different concentrations (5, 10, 20, 40μM) of icariin on HIF-1 in hepatoma cells. α influence of expression. like Figure 16 shown that with the increase of icariin concentration, its effect on HIF-1 in HepG2 cells under hypoxia α The inhibition of expression is enhanced.

Embodiment 3

[0112] Take 250mg of ethyl oleate-tributyrin (mass ratio 1:1) and add it to 600mg of surfactant HS-15, then take 160mg of co-surfactant PEG-400 into the above mixed system, and then take 17mg of Carriin was added to the above-prepared mixed system, and magnetically stirred for 2 hours until no insoluble matter was suspended in the system. Drop into the mixed system prepared above, and continuously stir the system at a constant speed until a transparent and clear system appears, which is the icariin magnetic microemulsion.

[0113] The ginseng polysaccharide was added to water, and the polysaccharide aqueous solution was obtained by magnetic stirring at 50 °C for 24 h. Take 140 mL of polysaccharide aqueous solution with a concentration of 20 mg / mL in a three-necked flask; add 1.4 g of N -Isopropylacrylamide, magnetic stirring for 0.5h, fully dissolved; remove the oxygen in the bottle by vacuuming, and add 7mL of 0.8% ammonium persulfate aqueous solution and 7mL of 0.8% tetrame...

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Abstract

The present invention relates to a traditional Chinese medicine composite thermosensitive gel embolic agent, a preparation method and application thereof. α (HIF‑1 α ), a mixed oil phase of tributyrin and ethyl oleate, a surfactant, a co-surfactant and water, and the Chinese medicine polysaccharide composite temperature-sensitive material is N -The hydrogel material obtained by the polymerization of isopropylacrylamide in the traditional Chinese medicine polysaccharide solution. The traditional Chinese medicine compound thermosensitive gel embolizing agent of the present invention can significantly enhance the embolic effect of the embolizing agent on the end of liver cancer blood vessels, promote the thrombus formation at the embolization site, and induce M1-type polarization and HIF-1 of tumor-associated macrophages (TAMs) by inducing α Its expression remodels the tumor immune microenvironment, improves the efficacy of interventional therapy for liver cancer, and reduces the risk of recurrence.

Description

technical field [0001] The invention belongs to the field of antitumor preparations, in particular to a traditional Chinese medicine compound thermosensitive gel embolic agent, a preparation method of the thermosensitive gel embolic agent, and an application in the preparation of a liver cancer embolization treatment agent. Background technique [0002] Transarterial embolization / chemoembolization (TAE / TACE) is the standard clinical treatment for middle-advanced primary liver cancer, which can block tumor blood supply through hepatic artery injection of embolic agents, resulting in ischemic necrosis. At present, TAE / TACE treatment still has the limitations of limited efficacy and easy recurrence. The important reasons are: (1) The existing embolic agents cannot completely block the blood vessels at the end of the tumor, which leads to the generation of collateral circulation and restores blood supply to the tumor site; ②Long-term ischemia and hypoxia at the site of postopera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/08A61L24/04A61L24/00A61L24/06A61L24/02A61K31/352A61K31/715A61K9/06A61K47/10A61K47/32A61K47/02A61P35/00A61P35/04A61K49/12
CPCA61L24/08A61L24/046A61L24/0015A61L24/06A61L24/02A61L24/0031A61L24/001A61K31/352A61K31/715A61K9/06A61K47/10A61K47/32A61K47/02A61P35/00A61P35/04A61K49/126A61L2300/416A61L2400/12A61L2300/216A61L2300/602C08L33/24C08L71/02C08L5/00A61K2300/00
Inventor 陈彦史新萌瞿鼎刘聪燕刘玉萍
Owner JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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