Surface plasma resonance sensing chip and preparation method thereof and application thereof

A surface plasmon and sensor chip technology, applied in ion implantation plating, coating, instrumentation, etc.

Active Publication Date: 2013-02-13
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no SPR sensor suitable for detecting lipopolysaccharide, and there is no real-time, fast, simple, quantitative and ultra-sensitive method for detecting the content of lipopolysaccharide in aqueous solution, especially the content of lipopolysaccharide in water for injection, using SPR technology

Method used

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  • Surface plasma resonance sensing chip and preparation method thereof and application thereof
  • Surface plasma resonance sensing chip and preparation method thereof and application thereof
  • Surface plasma resonance sensing chip and preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] A surface plasmon resonance sensing chip, comprising a glass substrate layer, a gold film layer, and a probe molecule layer; a gold film layer is arranged on the glass substrate layer, and a probe molecule layer is arranged on the gold film layer; the gold film layer The thickness of the probe molecule layer is 10-60nm; the thickness of the probe molecule layer is 1-100nm; the probe molecule layer is a layer of one or two or more probe molecules in the following structures:

[0070]

[0071] In the formula, Ar 1 Thiophene, pyrrole, benzene, naphthalene, anthracene, pyrene, indole, coumarin, fluorescein, carbazole, rhodamine, cyano dyes, fluorene or quinoline, respectively;

[0072] Ar 2 One of the following structures respectively:

[0073]

[0074] X, Y, W are O, S, N-R respectively 5 or Si-R 6 R 7 ;

[0075] Z 1 ,Z 2 , R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 , R 14 , R 15 Hydrogen atom; 1-18 carbon a...

Embodiment 2

[0079] A method for preparing a surface plasmon resonance sensor chip, the specific steps are:

[0080] 1) Using vacuum evaporation technology, a thickness of 50nm gold is plated on the surface of the glass substrate;

[0081] 2) The glass substrate coated with the gold film obtained in step 1) is completely immersed in the probe molecule (PT1) solution with a concentration of 0.01mg / mL, and the solvent is N,N-dimethylformamide, Place at room temperature for 1 hour;

[0082] 3) After soaking completely, take out the glass substrate, rinse the glass substrate repeatedly with double distilled water to obtain a chip, and store it in double distilled water for later use.

[0083] figure 2 An atomic force microscope (AFM) image of a gold film coated on a glass substrate. (a) Gold film surface, 5um range; (b) Gold film surface, 1um range. figure 2 The white particles in (C) are probe molecules, and their coverage is about 20%.

[0084] The surface plasmon resonance sensor chi...

Embodiment 3

[0087] A method for preparing a surface plasmon resonance sensor chip, the specific steps are:

[0088] 1) Using magnetron sputtering technology, the thickness of 50nm gold is plated on the surface of the glass substrate;

[0089]2) The glass substrate plated with gold film obtained in step 1) is completely immersed in the probe molecule (PT1) solution with a concentration of 10 mg / mL, the solvent is acetonitrile, and placed at room temperature for 10 hours;

[0090] 3) After soaking completely, take out the glass substrate, rinse the glass substrate repeatedly with double distilled water to obtain a chip, and store it in double distilled water for later use.

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Abstract

The invention discloses a surface plasma resonance sensing chip which comprises a glass substrate layer, a gold film layer and a probe molecule layer. The gold film layer is arranged on the glass substrate layer, and the probe molecule layer is arranged on the gold film layer. The invention further discloses a preparation method of the surface plasma resonance sensing chip. A resonance spectrum technology is utilized to process surface plasma generated from the surface of a gold film on a glass substrate, and therefore the content of lipopolysaccharide in aqueous solution can be detected quickly, simply, conveniently, quantitatively and ultra-sensitively.

Description

technical field [0001] The invention belongs to the field of surface plasmon sensor chip preparation, and in particular relates to a surface plasmon resonance sensor chip that can be used for lipopolysaccharide detection and its preparation method and application. Background technique [0002] Lipopolysaccharide (LPS for short) is a polymer composed of lipids and polysaccharides connected by covalent bonds. It is a major component of the outer membrane of Gram-negative bacteria and is a potent bacterial toxin known as endotoxin. Lipopolysaccharide is released when Gram-negative bacteria such as E. coli and Salmonella enterica multiply or lyse. Gram-negative bacteria in the human body release a large amount of lipopolysaccharide in the cell wall, which will cause infection and inflammation in the human body, and will further develop into a major disease that seriously threatens human health-sepsis. Due to the high toxicity of lipopolysaccharide, in order to effectively cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C03C17/38G01N21/55G01N21/552
CPCG01N21/553C03C17/38G01N33/487C23C14/18C03C17/09C03C2217/255C03C2218/151C03C2218/156
Inventor 张洪艳蓝敏焕汪鹏飞
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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