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Separation and purification method for erodium stephanianum willd element in erodium stephanianums and application of separated highly-purified erodium stephanianum willd element

A purification method, the technology of Zhonglaohe, which is applied in chemical instruments and methods, preparations for skin care, preparation of sugar derivatives, etc., to achieve the effect of high efficiency and simple method

Inactive Publication Date: 2013-02-13
嘉兴纳诺生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there has been no public report on the effective purification and application of the truly active geraniin in G.

Method used

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  • Separation and purification method for erodium stephanianum willd element in erodium stephanianums and application of separated highly-purified erodium stephanianum willd element
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  • Separation and purification method for erodium stephanianum willd element in erodium stephanianums and application of separated highly-purified erodium stephanianum willd element

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Cultivation of guary grass:

[0024] In March-April when the mid-day temperature is about 15 degrees, sow 3-5 seeds of geranium in the cups for planting, and transplant the 6 cm seedlings that grow after 4 weeks to the plastic-covered cups. In the field of film (130 centimeters in width, 30 centimeters in height, and 25 meters in length), there are 6 plants in each column, and 15 centimeters between plants are planted. After planting, 5-6 plants were picked every 2 weeks, and the weight and content of geranine in each part of each plant were measured. The results are shown in Table 1. According to the results in Table 1, among the various parts, the leaves contain the highest content of geranin. In addition, the most fresh weight is harvested after 11 weeks after planting. It is the best time, and the leaves contain the highest active ingredients. .

[0025] Table 1 The weight and content of geranidin (%) of fresh geranium after planting

[0026]

Embodiment 2

[0028] Separation and purification of geranitin in geranium:

[0029] Take 2.5kg of geranium harvested 11 weeks after planting, 50L of water and 100g-300g of citric acid in a stainless steel container (50L), heat at 60-80°C for 1-3 hours while stirring, and cool to 25°C. The dross was removed with a wire mesh (30 mesh), and 50 L of the extract was obtained by filtration with a filter paper filter (pore diameter: 1 μm). This extract is processed through a synthetic resin (Mitsubishi Chemical, Diamond Ion HP-20, 2000ml) method. Activation (2000ml of 4%NaOH water is passed through at a speed of 33ml / min, and washed with 6000ml of water. Then, 4%H 2 SO 4 2000ml of water is passed through at a speed of 33ml / min, and then washed with 6000ml of water), and 50L of extract is put into a HP-20 type chromatographic column, and after passing through at a speed of 33ml / min, it is washed with 6000ml of water wash. The dissolution method of geranitin containing active ingredients in ger...

Embodiment 3

[0032] Melanin production inhibiting effect test

[0033] We all know that the main cause of spots and freckles is melanin, and the formation of melanin through ultraviolet rays is a way for the skin to protect itself. On the other hand, the reasons for the formation of spots and freckles have also been clarified. Melanin is formed by the conversion of tyrosine L-DOPA into DOPA quinone and the condensation of DOPA quinone. Associated with these two transformation reactions is tyrosinase. Pine and other authors have published (M.MATUKI et.al.YAKUGAKU ZASSHI128(8)1203-1207,2008) the report that glutathione can prevent the formation of melanin by hindering the condensation reaction of DOPA quinone. In the present invention, it can be confirmed that L-DOPA is transformed into DOPA quinone by tyrosinase, and then DOPA quinone is condensed to become melanin, which also shows that high-purity geranium can hinder the condensation of DOPA quinone and thus hinder melanin form.

[003...

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Abstract

The invention provides a separation and purification method for erodium stephanianum willd element in erodium stephanianums. The separation and purification method includes the following steps: extracting the erodium stephanianums in water solution with organic acid or alcohol water solution or methanol water solution, carrying out purification in one of supercritical extracting method, membrane processing method, dialysis membrane processing method, recrystallizaiton processing method, centrifugal separation method, synthetic resin absorption method and ion exchange resin method, and the like to obtain highly-purified erodium stephanianum willd element. The active ingredient, namely the erodium stephanianum willd element in the erodium stephanianum willd can be extracted out without being decomposed. The separation and purification method is simple in practicability and high in efficiency. The separated high-purity erodium stephanianum willd element has functions of an ultrastrong antioxidant effect, melanin forming preventing effect, specific enzyme activity blocking effect, superoxide dismutase (SOD) activity effect, and the like, so the extracted high-purity erodium stephanianum willd element can be widely applied to skin whitening cosmetics, functional foods, health foods, pharmaceuticals, quasi-drugs, underarm deodorants and the like.

Description

technical field [0001] The present invention relates to a high-purity separation and purification method of effective active ingredients contained in geranium, and the active ingredients obtained by using the method are used in functional foods, food raw materials, food additives, health foods, pharmaceuticals, medical skin care products , and cosmetic applications. Background technique [0002] Geranium nepakense Scientific name: Geranium nepakense Sweet sunsp.Thunbergii (Sieb.et Zucc.Hara), is a perennial herb of the genus Geranium, one of the representative herbs in China and Japan, generally in It is mainly wild in the mountains and fields. The upper part of it is naturally dried and boiled with water to relieve diarrhea, prevent constipation, and clear away heat and detoxify. Because of its good effect, it has been widely used among the people. In addition, it has been reported in the literature that the refined liquid separated by water and alcohol can be used in anti...

Claims

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Application Information

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IPC IPC(8): C07H13/08C07H1/08A61Q19/02A61Q15/00A61P39/06
CPCY02P20/54
Inventor 钟旭东市龙太
Owner 嘉兴纳诺生物工程有限公司
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