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Blood sample collection card for disease screening and kit comprising same

A collection card and kit technology, which is applied in the field of blood sample collection cards for disease screening and kits containing the blood sample collection cards, can solve problems such as high price, and achieve low cost, long storage time and simple operation. Effect

Inactive Publication Date: 2013-02-13
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Blood sample collection card is one of the main carriers for storing blood samples for neonatal disease screening. At present, most of them use imported FTA cards or imported 903 filter paper as carriers, which are expensive and subject to foreign sources. In order to avoid the shortage of imported goods and affect our country The blood sample collection for neonatal disease screening is carried out, and it is urgent to develop a blood sample collection card for genetic diseases with easy-to-obtain and low-cost raw materials and a DNA extraction method suitable for it

Method used

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  • Blood sample collection card for disease screening and kit comprising same
  • Blood sample collection card for disease screening and kit comprising same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Blood sample collection card for genetic disease screening and its preparation

[0044] The blood sample collection card consists of two parts, that is, the adsorption material used to absorb the blood sample and the paper containing the personal information registration form, blood collection method and instructions for precautions. The absorbent material is attached to one side of the paper, and four circular collection circles with a diameter of 1.5 cm are printed on the absorbent material. This printing process facilitates the complete and pollution-free removal of blood sample specimens from the filter paper.

[0045] The absorbent material used to absorb blood samples can be any sheet material that can absorb blood, including various paper and cloth materials, such as cotton cloth, writing paper, qualitative filter paper, quantitative filter paper, 903# filter paper or sanitary paper Wait. The personal information registration form is partially folded ...

Embodiment 2

[0047] Example 2 Containing the kit for blood sample DNA extraction containing blood sample collection card

[0048] Kit composition:

[0049] 1. The blood sample collection card of Example 1;

[0050] 2. Tissue digestion solution: 8mM Tris-HCl+8mM EDTA+8mM NaCl+0.5%SDS;

[0051] 3. LiCl solution 6.5mol / l;

[0052] 4. Proteinase K 20mg / ml;

[0053] 5. TE solution: 10mM Tris-HCl+0.1mM EDTA.

Embodiment 3

[0054] Example 3 The kit containing the blood sample collection card is used to extract blood sample DNA

[0055] Utilize the kit containing blood sample collection card of embodiment 2 to carry out blood sample DNA extraction, operation is as follows:

[0056] 1) Collect blood samples with a blood sample collection card, about 50 μl per piece, and store at room temperature;

[0057] 2) Put one piece of dried blood into a 1.5ml centrifuge tube;

[0058] 3) Add 350 μl of tissue digestion solution to the centrifuge tube, then add 5 μl of proteinase K, and shake for 5 seconds with a vortex mixer;

[0059] 4) Digest in a water bath at 50°C for 2 hours, and invert and mix well when digesting for 30 minutes and 60 minutes respectively;

[0060] 5) After 2 hours of digestion, add 300 μl LiCl solution to each tube, shake with a vortex mixer for 5 seconds, and place in ice bath for 10 minutes;

[0061] 6) Centrifuge at 13000 rpm for 10 minutes, take 500 μl of supernatant and transfer ...

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Abstract

The invention provides a blood sample collection card for disease screening. The blood sample collection card comprises an adsorption material for adsorbing a blood sample and paper containing personal information registration, blood sampling methods and matters needing attention. The adsorption material is adhered to one side of the paper, and a sampling ring is printed on the adsorption material which is selected from cotton fabrics, writing paper or sanitary paper, and the like. The invention further provides a kit comprising the blood sample collection card and used for extracting deoxyribonucleic acid (DNA) in the blood sample. By means of the blood sample collection card and the kit, the DNA in the blood sample can be extracted, and accordingly relevant genetic testing can be performed. The blood sample collection card for disease screening and the kit comprising the blood sample collection card have the advantages that the sample can be preserved at the room temperature, and low temperature storage conditions are not needed; the sample stored on the blood sample collection card can be preserved at the room temperature and can still be used for relevant genetic testing within 20 years, and the preservation time is long; the sample can be transported quickly and conveniently and is suitable for post express transportation in remote and border areas; the required blood sample is few, and generally speaking, only 50mul of blood is enough; and the DNA extraction effect of the blood sample is good, operation is simple and cost is low.

Description

technical field [0001] The invention relates to a blood sample collection card used for screening of diseases, especially hereditary diseases, and a kit containing the blood sample collection card. Background technique [0002] Neonatal disease screening refers to the discovery of certain serious congenital genetic metabolic diseases through laboratory testing techniques for newborn babies, so as to make early diagnosis and early treatment, and prevent the baby from causing brain, liver, kidney and other damages to intelligence. , physical disability and even death. [0003] Blood sample collection card is one of the main carriers for storing blood samples for neonatal disease screening. At present, most of them use imported FTA cards or imported 903 filter paper as carriers, which are expensive and subject to foreign sources. In order to avoid the shortage of imported goods and affect our country With the blood sample collection for neonatal disease screening, it is urgent...

Claims

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Application Information

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IPC IPC(8): C12M1/26C12Q1/24
Inventor 张志栋杨君黄茜易翔
Owner BIOSINO BIO TECH & SCI
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