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Wave generation type cell bioreactor

A technology of cell biology and reactor, which is applied in the field of bioengineering, can solve the problems of unsatisfactory suspension or static culture effect of microcarrier-adhered cells, bottlenecks in cell culture scale, and poor suspension culture effect of non-adherent cells. Eliminate the effect of cell shearing, simple structure, mild effect

Active Publication Date: 2014-09-17
WUHAN CHOPPER BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, Braun in Germany and Applikon in the Netherlands adopt mechanical stirring paddles and bubbling ventilation mode, which are often used for suspension culture of non-adherent animal cells, but the suspension or static culture of microcarrier-adhered cells is not ideal; American NBS , Hangzhou Anpu respectively adopt the lift-type stirring paddle microcarrier system and the torrent reactor disposable paper carrier system, which are used for microcarrier suspension culture and static culture of adherent cells. The suspension culture effect of parietal cells is not good, and there is a bottleneck in the scale of cell culture in Hangzhou Anpu Bioreactor

Method used

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  • Wave generation type cell bioreactor
  • Wave generation type cell bioreactor
  • Wave generation type cell bioreactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Below in conjunction with accompanying drawing, the present invention is further described: as Figure 1-4 As shown, a bulging cell bioreactor includes an inverted cone-shaped bulging stirring paddle 7 formed by rotating an inverted right-angled triangle whose hypotenuse is an arc along the right-angled side.

[0028] The hypotenuse of the arc of the inverted conical bulging stirring paddle 7 is a circular arc.

[0029] A drum wave type cell bioreactor, which also includes a tank body 9, a tank cover 8, a motor 1, a rotating shaft 2, a rotor 3 and a shaft sleeve 4, and the shaft sleeve 4 and the inverted conical drum wave stirring paddle 7 are fixed together through the end surface , the periodic up and down movement of the shaft sleeve 4 drives the up and down movement of the drum stirring paddle 7, because the drum stirring paddle 7 has a certain volume and its shape is an inverted cone, when the drum wave stirring paddle moves downward, it will move the liquid along ...

Embodiment 2

[0040] Below in conjunction with accompanying drawing, the present invention is further described: as Figure 1-4 As shown, a bulging cell bioreactor includes an inverted cone-shaped bulging stirring paddle 7 formed by rotating an inverted right-angled triangle whose hypotenuse is an arc along the right-angled side.

[0041] The hypotenuse of the curved line of the inverted conical drum stirring paddle 7 is an elliptical arc.

[0042] A drum wave type cell bioreactor, which also includes a tank body 9, a tank cover 8, a motor 1, a rotating shaft 2, a rotor 3 and a shaft sleeve 4, and the shaft sleeve 4 and the inverted conical drum wave stirring paddle 7 are fixed together through the end surface , the periodic up and down movement of the shaft sleeve 4 drives the up and down movement of the drum stirring paddle 7, because the drum stirring paddle 7 has a certain volume and its shape is an inverted cone, when the drum wave stirring paddle moves downward, it will move the liqui...

Embodiment 3

[0053] Below in conjunction with accompanying drawing, the present invention is further described: as Figure 1-4 As shown, a bulging cell bioreactor includes an inverted cone-shaped bulging stirring paddle 7 formed by rotating an inverted right-angled triangle whose hypotenuse is an arc along the right-angled side.

[0054] The hypotenuse of the curved line of the inverted conical drum stirring paddle 7 is an elliptical arc.

[0055] A drum wave type cell bioreactor, which also includes a tank body 9, a tank cover 8, a motor 1, a rotating shaft 2, a rotor 3 and a shaft sleeve 4, and the shaft sleeve 4 and the inverted conical drum wave stirring paddle 7 are fixed together through the end surface , the periodic up and down movement of the shaft sleeve 4 drives the up and down movement of the drum stirring paddle 7, because the drum stirring paddle 7 has a certain volume and its shape is an inverted cone, when the drum wave stirring paddle moves downward, it will move the liqui...

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Abstract

The invention relates to a wave generation type cell bioreactor which comprises an inverted-cone-like wave generation stirring paddle (7) formed by rotating an inverted right triangle with an arc bevel edge along a right triangle edge. The arc bevel edge of the inverted-cone-like wave generation stirring paddle (7) comprises an arc, an elliptic arc and the like. The wave generation type cell bioreactor has the advantages of simple structure, wide applicability and strong expandability. The wave generation type cell bioreactor can be used for cultivating pure suspension cells and microcarrier adherent cell at the same time, and can flexibly adjust the structure and the operating mode of the paddle according to the cultivation modes of different cells. The wave generation type cell bioreactor is easy to amplify, and can easily realize the amplification cultivation on the size of the animal cell size. The wave generation type cell bioreactor creates conditions for the large-scale animal cell cultivation and the industrial biology and tissue product production.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a billow-type cell bioreactor for cell culture. Background technique [0002] Bioreactor is the key equipment in the process of animal cell culture. It aims to artificially design a suitable growth environment for cells so that they can proliferate rapidly in vitro to obtain the biological products that people need. Since animal cells are quite different from microorganisms in terms of their morphology, growth mode, nutritional requirements, and liquid thermodynamic environment, it can be said that animal cell culture has stricter requirements for bioreactors, and classic microbial reactors are obviously not applicable. Due to the needs of animal cell culture and tissue engineering, this prompts the research and development of new animal cell bioreactors. [0003] Animal cells have no cell wall and have low environmental tolerance. Compared with bioreactors for microbial ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/02
Inventor 李伟秦红刚漆世华谢红玲韩兴温文生王桢桢戟治旭陈志刚
Owner WUHAN CHOPPER BIOLOGY