Method for producing hydrocarbons for making fuel by using sea water as medium and special strain

A seawater and recombinant bacteria technology, applied in the biological field, can solve the problems of resource consumption, environmental pollution, difficult to treat wastewater, etc., and achieve the effects of low cost, simple nutritional requirements, and good industrial application prospects.

Active Publication Date: 2013-02-13
北京微构工场生物技术有限公司
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Problems solved by technology

This method of using food crops as raw materials to produce biofuels has produced a situation of competing with people for food and occupying a large amount of arable land; the traditional fermentat

Method used

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  • Method for producing hydrocarbons for making fuel by using sea water as medium and special strain
  • Method for producing hydrocarbons for making fuel by using sea water as medium and special strain
  • Method for producing hydrocarbons for making fuel by using sea water as medium and special strain

Examples

Experimental program
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Example Embodiment

[0061] Example 1. Isolation, identification and growth characteristics of Halomonas sp. LS 21

[0062] 1. Isolation of strain LS 21

[0063] Soil and water samples were obtained from the Dabancheng Salt Lake in Xinjiang, and strains were isolated from them using cellulose as the sole carbon source. Five strains that can produce and utilize cellulose as the sole carbon source for growth were obtained, and one of the PHA strains that grew well and could accumulate was selected and named LS 21.

[0064] Production of cellulose culture medium: filter the straw cellulose soaking solution with gauze to filter out the residual straw, add NaCl (40g / L) and the mixed solution of component I and trace elements in proportion, adjust the pH value to 10 with NaOH solution, After the precipitate is separated, centrifuge at 5000 rpm for 5 min, and reserve the supernatant as the straw cellulose medium.

[0065] Straw cellulose soaking solution: Weigh 6 g of the straw after gas explosion treatment (th...

Example Embodiment

[0083] Example 2. Application of Halomonas sp. LS 21CGMCC No.6593 in fermentation using seawater as a medium

[0084] 1. Preparation of fermentation medium with seawater

[0085] The use of seawater and kitchen waste is cost-saving, energy-saving and environmentally friendly. Therefore, it can be used as a raw material for the culture medium. Based on the above-mentioned purpose, a fermentation medium using seawater as a medium is developed: natural seawater can be used, or the same or the same components A very similar artificial seawater formula (Mocledon formula) configuration simulation; the organic waste in the kitchen industry contains about 79% of water, about 29% of crude fat in dry materials, about 20% of crude protein, and 31% of starch. Mixing can be used C source, N source and corresponding ratio simulation.

[0086] Natural sea water (NaCl content is about 26g / L), the main ion content in the fermentation medium used to configure the fermentation medium is as follows: + ...

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Abstract

The invention discloses a method for making fuel by using sea water as a medium and a special strain. The special strain is holomonas (Halomonas sp.)LS21, of which the collection number is CGMCCNo.6593. Experiments show that the Halomonas sp.LS21 and the recombinant strain thereof can produce hydrocarbons mainly comprising alkane, olefin and long-chain fatty acid by using natural sea water as a fermenting medium and using cheap carbon materials and nitrogen sources such as pre-treated cellulose, hemicellulose, waste oils and fats and kitchen waste as substrate. The separated hydrocarbons can be used as biofuel, and continuous fermentation without sterilization can be realized. The holomonas and the recombinant strain thereof have low requirement on nutrients. The fermentation process can go continuously without sterilization. The method is simple and easy to control and low in cost, and has a great prospect in industrial application.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing hydrocarbons for fuel using seawater as a medium and a special strain. Background technique [0002] For centuries, conventional fossil fuels have supported the development of industrial civilization. However, due to the non-renewability of fossil energy, the precious resources accumulated by the earth for hundreds of millions of years will eventually be exhausted under the intensive exploitation and consumption of human beings. At the same time, problems such as climate change and environmental pollution caused by the excessive use of fossil resources will also make human beings pay a huge price. The problem of energy supply will become an important factor restricting the sustainable development of my country's society and economy in the future. [0003] In order to ensure the independence and sustainability of energy supply, countries have set off a new wav...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/62C12P7/42C12P7/64C12N1/21C12P5/02C12R1/01
Inventor 陈国强岳海涛尹进
Owner 北京微构工场生物技术有限公司
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