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Microtopological-structure plate flow chamber capable of applying electric and shearing force stimulation

A technology of flat flow chamber and topological structure, which is applied in the field of flat flow chamber to achieve the effect of simple and compact structure

Active Publication Date: 2014-06-25
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no flow chamber that can control the microtopography while applying electrical and shear force stimulation to the cells.

Method used

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  • Microtopological-structure plate flow chamber capable of applying electric and shearing force stimulation
  • Microtopological-structure plate flow chamber capable of applying electric and shearing force stimulation
  • Microtopological-structure plate flow chamber capable of applying electric and shearing force stimulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Depend on figure 1 , figure 2 , image 3 and Figure 4 It can be seen that the plate flow chamber capable of implementing multi-directional electrical stimulation of the present invention is composed of three layers of plates and two layers of gaskets. A rectangular slit 6 is provided and the lower gasket 10 is provided with a flow cell 18 . The width and length of the flow chamber 18 should be much larger than the difference between the thickness of the flow chamber 18 and the thickness of the electrode plate 16 placed therein. The upper plate 2 , the middle plate 5 and the lower plate 11 can be made of materials such as polycarbonate, resin glass or metal, and the upper gasket 3 and the lower gasket 10 can be made of silicone resin or the like. like Figure 5 , Image 6 and Figure 7 It can be seen that the culture liquid enters the triangular diversion groove 4 in the upper gasket 3 from the upper plate 2 and the upper conduit 1, so that the fluid is fully di...

Embodiment 2

[0045] The micro-topological structure on the electrode plate 16 of the present invention is not limited to the micro-grooves provided in Embodiment 1, and micro-protrusions of various shapes such as triangles, quadrangles, pentagons, hexagons or cylinders can also be formed on the silicon wafer. array. Taking a square boss as an example, Figure 10 It is a top view of the electrode plate on the bottom surface of the flow chamber of the flat flow chamber with micro-topography that can apply electrical and shear force stimulation, Figure 11 Yes Figure 10 The electrode plate on the bottom surface of the flow chamber of the flat plate flow chamber with a microtopological structure that can apply electrical and shear force stimulation is shown along the Figure 10 Sectional view of line E-E in . In order to obtain the electrode plate as shown in the figure, a square micro-protrusion array can be formed on a silicon wafer. The side length of the square micro-protrusion platfor...

Embodiment 3

[0047] The electrode plate 16 of the present invention can be prepared as a flat plate with uniform thickness to realize uniform fluid shearing force. The electrode plate 16 can also be prepared as a wedge-shaped plate with a uniform thickness change, so as to realize continuous shearing force. Figure 12 is a cross-sectional view of an electrode plate 16 whose thickness varies uniformly, Figure 13 Yes Figure 12 A side view of the bottom electrode plate 16 is shown. When preparing the electrode plate 16 with a uniform thickness change, the thickness of the PDMS film 23 can be kept uniform, and the thin slice 22 can be made into a wedge shape with an inclination of 5-60°. The slope of the wedge. For the convenience of processing, it is preferable to keep the thickness of the PDMS film 23 uniform, and make the thin slice 22 into a wedge shape with an inclination of 5-60°. And other methods and structure are all identical with embodiment 1 or embodiment 2.

[0048] The usa...

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Abstract

The invention discloses a microtopological-structure plate flow chamber capable of applying electric and shearing force stimulation, which is formed by superposing three plates and two gaskets, wherein an upper plate (2) is provided with a fluid inlet / outlet port; an upper gasket (3) is provided with a diversion trench (4); a middle plate (5) is provided with a rectangular slit (6); a lower gasket (10) is provided with a flow cavity (18); the middle plate (5) above the flow cavity (18) is provided with a plate electrode (7); a lower plate (11) below the flow cavity (18) is provided with a plate electrode (16); a pair of electrodes (14 and 17) are respectively arranged on four sides of the flow cavity (18); and the plate electrode (16) is composed of a sheet (22), a PDMS (polydimethylsiloxane) film (23) and a graphene film (24), and has a microtopological structure on the surface. When applying electric and shearing force stimulation with different modes and intensities, the invention implements microtopological structure regulation on the cell (19). The structure of the device is simple, compact and reliable.

Description

technical field [0001] The invention relates to a flat plate flow chamber, in particular to a flat plate flow chamber with a micro topological structure which can be stimulated by electricity and shear force. Background technique [0002] Tissue engineering is based on the principles of cell biology and engineering, combining seed cells, biological scaffolding materials and growth factors to construct tissues and organs in vitro / in vivo to maintain, repair, regenerate or improve the function of damaged tissues and organs. The biological scaffold material of compound seed cells is the core of tissue engineering. Numerous studies have shown that the matrix microtopology, electrical stimulation and shear force can regulate the biological functions of cell adhesion, proliferation and differentiation, and can promote the relationship between seed cells and The interaction of scaffold materials regulates the secretion of extracellular matrix in cells. At present, in tissue engine...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42
CPCC12M23/12C12M23/20C12M35/02C12M21/08C12M23/04
Inventor 吕永钢董单娟邹杨
Owner CHONGQING UNIV