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Method for improving efficiency of meso-porous silicon nano materials entering into cells by utilizing cationic liposome

A technology of nanomaterials and liposomes, which is applied in the direction of introducing foreign genetic material, pharmaceutical formulations, and genetic material components using carriers, can solve problems such as low efficiency and achieve the effect of improving efficiency.

Inactive Publication Date: 2014-03-05
NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the surface of the cell membrane is negatively charged, the efficiency of mesoporous silicon, which is also negatively charged on the surface, entering the cell is particularly low.

Method used

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  • Method for improving efficiency of meso-porous silicon nano materials entering into cells by utilizing cationic liposome
  • Method for improving efficiency of meso-porous silicon nano materials entering into cells by utilizing cationic liposome
  • Method for improving efficiency of meso-porous silicon nano materials entering into cells by utilizing cationic liposome

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Embodiment Construction

[0016] In order to better understand the technical content of the present invention, specific embodiments are given together with the attached drawings for description as follows.

[0017] Positive liposome Lipofectamine2000 is a bilayer hydrophobic liposome material, which is currently routinely used to transduce DNA into cells. like figure 1 As shown, since the surface of liposomes is positively charged, it can surround the negatively charged mesoporous silicon (MSNs) on the surface. Carry mesoporous silicon into the cell.

[0018] Based on the above principles, the present invention proposes a method for utilizing positive liposomes to increase the efficiency of mesoporous silicon nanomaterials entering cells, comprising the following steps:

[0019] Dilute and dissolve 20 microliters of positive liposomes with 500 microliters of serum-free medium, the dilution ratio is 1:25, and let stand at room temperature for 5 minutes;

[0020] Dilute and dissolve 0.5 mg of mesoporo...

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Abstract

The invention provides a method for improving efficiency of meso-porous silicon nano materials entering cells by utilizing cationic liposome. The method comprises the following steps of utilizing 500mu l of serum-free culture medium to dilute and dissolve 20mu l of the cationic liposome, and standing for 5 minutes at the room temperature, wherein the dilution rate is 1:25; utilizing 500mu l of serum-free culture medium to dilute and dissolve 0.5mg of meso-porous silicon nano materials, and standing for 5 minutes at the room temperature; mixing the diluted and dissolved cationic liposome with the diluted and dissolved meso-porous silicon nano materials, and standing for 20 minutes at the room temperature, wherein the diluted and dissolved cationic liposome and the diluted and dissolved meso-porous silicon nano materials have equal quantity; and evenly adding the mixed solution into 4ml of culture cells containing 1640 culture medium, slightly mixing, and culturing the culture cells in a carbon dioxide incubator at the temperature of 37 DEG C. The method is used for improving efficiency of meso-porous silicon nano materials, especially meso-porous silicon with negative charge on the surface entering the cells, so that the meso-porous silicon nano materials can play biological effect.

Description

technical field [0001] The invention relates to the enhancement of the entry of mesoporous silicon nanomaterials into cells, and in particular to a method for increasing the entry efficiency of mesoporous silicon nanomaterials into cells by using positive liposomes. Background technique [0002] Nanomaterials is a rapidly developing field, and mesoporous silicon (MSN) is a nanomaterial. Mesoporous silicon has the advantages of porosity, large surface area, and easy chemical modification of the surface. In recent years, people have studied the use of mesoporous silicon to load drugs for tumor chemotherapy, directly enter the target cells through endocytosis, reach the intracellular lysosome under acidic pH conditions, release the loaded drugs, and achieve the effect of killing tumor cells. Therefore, the toxic effect of the drug on normal cells before reaching the tumor cells is avoided, and the effect of specifically killing tumor cells is exerted. Since the surface of mes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/02A61K48/00C12N15/63
Inventor 王建东卢光明
Owner NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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