Method for improving efficiency of meso-porous silicon nano materials entering into cells by utilizing cationic liposome
A technology of nanomaterials and liposomes, which is applied in the direction of introducing foreign genetic material, pharmaceutical formulations, and genetic material components using carriers, can solve problems such as low efficiency and achieve the effect of improving efficiency.
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[0016] In order to better understand the technical content of the present invention, specific embodiments are given together with the attached drawings for description as follows.
[0017] Positive liposome Lipofectamine2000 is a bilayer hydrophobic liposome material, which is currently routinely used to transduce DNA into cells. like figure 1 As shown, since the surface of liposomes is positively charged, it can surround the negatively charged mesoporous silicon (MSNs) on the surface. Carry mesoporous silicon into the cell.
[0018] Based on the above principles, the present invention proposes a method for utilizing positive liposomes to increase the efficiency of mesoporous silicon nanomaterials entering cells, comprising the following steps:
[0019] Dilute and dissolve 20 microliters of positive liposomes with 500 microliters of serum-free medium, the dilution ratio is 1:25, and let stand at room temperature for 5 minutes;
[0020] Dilute and dissolve 0.5 mg of mesoporo...
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