Method for testing dual-wavelength of light splitting luminosity

A detection method and a spectrophotometric technology, applied in the field of spectral analysis, can solve the problem of not being able to find the satisfaction

Active Publication Date: 2013-03-13
SINOCAT ENVIRONMENTAL TECH
View PDF3 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many components that are difficult to meet these conditions, so this method cannot be used
For exampl

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for testing dual-wavelength of light splitting luminosity
  • Method for testing dual-wavelength of light splitting luminosity
  • Method for testing dual-wavelength of light splitting luminosity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Preparation of platinum standard solution standard curve

[0078] Platinum standard solution: platinum concentration = 100μg / ml

[0079] Take 0.00, 1.00, 2.00, 3.00, 4.00, 5.00ml of platinum standard solutions respectively in a 25ml volumetric flask, add 10ml of 1mol / L stannous chloride solution, dilute to the mark with hydrochloric acid with a concentration of 1+6 , heat in a boiling water bath for 30 minutes, take it out to cool, and shake well. Use a 1cm cuvette, take the reagent blank as the reference solution, and measure the absorbance A at wavelengths of 420nm and 475nm by dual-wavelength photometry. 420 、A 475 .

[0080] Platinum working curve: Draw the working curve with the amount of platinum in m (μg) as the ordinate and the corresponding absorbance value ΔA as the abscissa. Calculate the slope K of the platinum working curve regression equation 1 and intercept b 1 .

[0081] Here, ΔA Pt =A 420 -s 2 A 475 -t 2 , coefficient s 2 and t 2 See rhodi...

Embodiment 2

[0085] Rhodium standard curve making

[0086] Rhodium standard solution: rhodium concentration = 50μg / ml

[0087] Take 0.00, 1.00, 2.00, 3.00, 4.00, 5.00ml of rhodium standard solutions respectively in a 25ml volumetric flask, add 10ml of stannous chloride solution with a concentration of 1mol / L, and dilute to the mark with hydrochloric acid with a concentration of 1+6 , heat in a boiling water bath for 30 minutes, take it out to cool, and shake well. Use a 1cm cuvette, take the reagent blank as the reference solution, and measure the absorbance A at wavelengths of 420nm and 475nm by dual-wavelength photometry. 420 、A 475 .

[0088] Rhodium working curve: draw the working curve with the amount of rhodium m (μg) as the ordinate and the corresponding absorbance value ΔA as the abscissa. Calculate the slope K of the rhodium working curve regression equation 2 and intercept b 2 .

[0089] Here, ΔA Rh =A 475 -s 1 A 420 -t 1 , coefficient s 1 and t 1 See platinum stand...

Embodiment 3

[0093] Platinum and rhodium content detection

[0094] Tin protochloride solution (1mol / L) configuration: take by weighing 22.6g tin protochloride (SnCl 2 2H 2 O), add (1+6) hydrochloric acid solution 100ml to dissolve and prepare.

[0095] Detect the test solution so that the amount of platinum and rhodium is within the measurement range, otherwise it will be diluted in proportion, take an appropriate amount of test solution and blank respectively in two 25ml volumetric flasks, add 10ml of a concentration of 1mol / L tin protochloride solution, and use a concentration of Dilute 1+6 hydrochloric acid to the mark, heat in a boiling water bath for 30 minutes, take it out to cool, and shake well. Use a 1cm cuvette, take the reagent blank as the reference solution, and measure the absorbance A at wavelengths of 420nm and 475nm respectively 420 , A475. The unit of absorbance is %.

[0096] In conjunction with embodiment 1, embodiment 2 platinum, rhodium standard curve and regres...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for testing dual-wavelength of light splitting luminosity. The method comprises the following steps of: preparing liquid to be tested and corresponding standard solution; selecting a corresponding largest absorbance as the testing wavelength; making a standard curve and a working curve of an object to be tested and an interference object; and calculating the content of the object to be tested according to an object (to be tested) content absorbance working curve regression equation that m is equal to KdeltaA+b. The method is strong in anti-interference capability, mutual interference of two components is eliminated, and the testing result is accurate; an absorbance calculation equation adopted is as follows: delta=A1-s2A2-t2; as the absorbance generated by the interference object is A1-s2A2-t2=0, the interference is eliminated, and the testing result is accurate; the method is simple and convenient, complex separation does not need to be carried out on two components which develop the color at the same time, so that the time, the labor and the agent are saved; two substances can be tested at the same time, and particularly the method has remarkable advantages for two components which are hard to be separated or cannot be separated; the method is applicable to more substances, and the method can be adopted only if the calculated deltaA is large enough, so that more substances can be tested with the attendance of a single interference object by using double wavelength.

Description

technical field [0001] The invention belongs to the technical field of spectroscopic analysis, in particular to a method for detecting the content of analytes in mixtures by means of dual-wavelength spectrophotometry. Background technique [0002] There are currently two calculation methods for measuring absorbance, one is the equal absorption formula, that is, ΔA=A1-A2; the other is the magnification formula: ΔA=KA 1 -A 2 . Among them, A 1 、A 2 is the absorbance at two wavelengths. The isoabsorption calculation can be attributed to the multiplier formula, that is, the case of K=1. [0003] Dual-wavelength spectrophotometry is a relatively advanced measurement method, and its principle is the Beer-Lambert theorem and the summation of absorbance. Dual-wavelength spectrophotometry eliminates interference, and the equal-absorption formula uses the same absorbance of the interfering component at two wavelengths, that is, A 1 -A 2 =0; the magnification formula uses the ab...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/31
Inventor 王科李晓婧杨路
Owner SINOCAT ENVIRONMENTAL TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap