Method for solving problem of bacterial variation degradation of liquid-strain nutrition liquid

A technology of liquid strains and liquid bacteria, which is applied in botany equipment and methods, horticulture, applications, etc., can solve the problems that have not been completely solved, such as bacterial variation and degradation, and achieve significant economic value and mild mutagenic effect

Active Publication Date: 2013-04-03
陕西众兴菌业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problem of bacterial variation and degradation has not been completely solved, especially there is no systematic solution to the problem of variation and degradation caused by various reasons

Method used

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  • Method for solving problem of bacterial variation degradation of liquid-strain nutrition liquid
  • Method for solving problem of bacterial variation degradation of liquid-strain nutrition liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Test materials

[0022] Strain Flammulina velutipes

[0023] Medium: (1) Seed medium: PDA medium. (2) Shake flask seed medium (%): soluble starch 4, glucose 1, peptone 0.3, KH 2 PO 4 0.1, magnesium sulfate heptahydrate 0.05, VB110ug / ml, VB 2 50ug / ml, pH6.5-6.7. (3) Shake flask fermentation medium (%): rice flour 5, glucose 1, peptone 0.5, KH 2 PO 4 , PH7.5.

[0024] 2. Degeneration performance: After 2 subcultures, the hyphae of Flammulina velutipes grow slowly and white, dense angular colonies; the growth of mycelia becomes thinner, and the production of fruiting body primordium decreases; the ability to produce asexual spores increases or decreases significantly .

[0025] 3. Solution:

[0026] (1) The percentage by weight added to the nutritional liquid is 3% itraconazole and 5% rapamycin;

[0027] (2) Store the bacteria in liquid nitrogen at ultra-low temperature;

[0028] (3) Separation of sexual spores is to select excellent fruiting bodies that meet...

Embodiment 2

[0031] 1. Strain Tricholomagambosum

[0032] Medium: (1) Seed medium: PDA medium. (2) Shake flask seed medium (%): soluble starch 4, glucose 1, peptone 0.3, KH 2 PO 4 0.1, magnesium sulfate heptahydrate 0.05, VB 1 10ug / ml, VB 2 50ug / ml, pH6.5-6.7. (3) Shake flask fermentation medium (%): rice flour 5, glucose 1, peptone 0.5, KH 2 PO 4 , PH7.5.

[0033] 2. Degradation performance: Tricholoma mycelium is getting thinner, less fungus balls, less burrs, the culture cycle is getting longer and longer, and more vacuoles are found during microscopic examination. When the strain is cultured on a slant, it produces abnormal pigments or changes from producing pigments to not producing pigments, or the amount of pigments produced increases or decreases significantly. During microscopic examination, a large number of hyphae changed from the original lock-like union to no lock-like union, and a large number of mononucleated hyphae appeared.

[0034] 3. Solution:

[0035] (1) Add ...

Embodiment 3

[0038] Embodiment 3 virus interferes with normal metabolism

[0039] 1. Degradation performance Lentinus edodes (Berk.) sing) strains are observed under the electron microscope to be infected with viruses to varying degrees, and the virus particles in the hyphae cells replicate in large numbers and the number increases sharply. It not only absorbs a lot of nutrients, but also Interfering with the normal metabolic activities of cells, causing mycelium activity to decline and growth to weaken.

[0040] 2. Cultivation of Test Tube Seeds

[0041] Formula: soybean flour 30-50%, corn dregs 10-30%, millet grains 5-15%, sorghum flour 5-15%, fresh eggs 10-30%;

[0042] Method: Take the materials with the above weight ratio, soak sorghum flour, millet grains, and corn dregs in water until there is no dry core, then remove them and mix them with soybean flour, fresh eggs, and then add water. The weight of water is 60-65% of the total weight of all materials. %, then fill the test tube ...

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Abstract

The invention relates to a method for solving a problem of bacterial variation degradation of liquid-strain nutrition liquid, in particular to a method for mushroom-liquid liquid strains. The method comprises a step of using an efficient mutagenic agent, wherein the efficient mutagenic agent comprises itraconazole and rapamycin, and the weight percentage of the itraconazole and the rapamycin in the nutrition liquid is respectively 0.5-5 percent and 1-7 percent. The method also comprises a step of low-temperature preservation strain, a step of adjusting culture conditions and a step of separation and purification. The method taking the itraconazole and the rapamycin as the efficient mutagenic agent as the core method, the mutagenesis is mild, and meanwhile, the strain preservation condition, the nutritional condition of the nutrition liquid, the passage number condition, the purification condition and the like are optimized. Experiments prove that the problem of bacterial variation degradation of various kinds of mushroom liquid-strain nutrition liquid can be basically solved, the method has an obvious economic value, and high-producing strain is obtained.

Description

technical field [0001] The invention belongs to the field of edible fungus strains, and in particular relates to a method for solving the variation and degradation of liquid strain nutrient liquid fungi. Background technique [0002] Strain degeneration usually refers to the phenomenon that one or more physiological traits and morphological characteristics of a strain gradually decrease or disappear after a long period of passage and preservation. The essence of strain degeneration is the variation of chromosome. In production practice, the problem of strain degradation is often encountered. Some strains have a decrease in fermentability (such as sugar and nitrogen consumption) or fecundity (such as spore production), and some have a decrease in the yield of fermented products. have very adverse effects. There are many reasons for the degradation of strains, but it must be distinguished from the morphological and physiological variations of strains caused by changes in cul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 陶军胡亚鹏
Owner 陕西众兴菌业科技有限公司
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