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Gonorrhoeae antigen detection method and detection kit and preparation method of detection kit

A detection kit and antigen detection technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of heavy workload, high environmental requirements, and long incubation time, and achieve the advantages of simple operation, high sensitivity, and convenient detection Effect

Active Publication Date: 2013-04-03
ZHUHAI ENCODE MEDICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the culture method has high sensitivity and strong specificity, the method has a large workload, takes a long time to cultivate, requires special facilities and professionals to operate, and has high requirements on the environment, requiring special instruments, and is subject to certain restrictions in actual clinical application.

Method used

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  • Gonorrhoeae antigen detection method and detection kit and preparation method of detection kit
  • Gonorrhoeae antigen detection method and detection kit and preparation method of detection kit

Examples

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Embodiment 1

[0031] In this embodiment, the method for detecting Neisseria gonorrhoeae antigen of the present invention includes the following steps:

[0032] a. The colloidal gold-labeled gonococcal antibody is loaded on a glass fiber carrier, and the detection carrier connected to the carrier of the gonococcal antibody is coated with a detection line made of the gonococcal antibody and an immunoglobulin Control line formed by protein G;

[0033] b. Take the specimens to be tested. In this embodiment, the specimens to be tested are female cervical secretions and male urethral specimens. The specimens to be tested are lysed and dropped on the carrier of the colloidal gold-labeled Neisseria gonorrhoeae antibody, such as If there is a Neisseria gonorrhoeae antibody forming the detection line and the control line on the detection carrier, it is positive, otherwise it is negative.

[0034] The gonococcal antibody is a gonococcal monoclonal antibody, or a gonococcal polyclonal antibody, or ...

Embodiment 2

[0053] The difference between this embodiment and the first embodiment: In this embodiment, the detection line 231 is composed of goat anti-gonococcal antibody, and the control line 232 is composed of rabbit anti-goat IgG antibody.

Embodiment 3

[0055] This embodiment is different from the first and second embodiments: in this embodiment, the detection line 231 is composed of rabbit anti-gonococcal antibody, and the control line 232 is composed of goat anti-rabbit IgG antibody.

[0056] The invention is applied to the field of Neisseria gonorrhoeae detection.

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Abstract

The invention discloses a gonorrhoeae antigen detection method and also discloses a detection kit for detecting a gonorrhoeae antigen and a method for preparing the detection kit. The gonorrhoeae antigen detection method comprises the following steps of: a, loading a colloidal gold labeled gonorrhoeae antibody on a glass fiber carrier; and b, dropping a split specimen to be detected on a carrier of the colloidal gold labeled gonorrhoeae antibody. The detection kit comprises a kit body (1) and a gonorrhoeae antigen colloidal gold labeled detection card (2), wherein a lysis solution A (3) and a lysis solution B (4) are stored in the kit body (1), and a specimen adding hole (11) and an observation hole (12) which are matched with the gonorrhoeae antigen colloidal gold labeled detection card (2) are formed in the kit body (1). The method for preparing the detection kit comprises the steps of: firstly, preparing the gonorrhoeae antigen colloidal gold labeled detection card (2) and then assembling. The invention is applied to the field of medicines.

Description

technical field [0001] The invention relates to a gonorrhea antigen detection method, a detection kit used in the method and a preparation method of the kit. Background technique [0002] Gonorrhea is one of the most common sexually transmitted diseases in China. Its pathogen is Neisseria gonorrhoeae, which is a serious human parasite. It exists in the purulent secretions of acute and chronic urethritis and neonatal conjunctival secretions. In men, it often manifests as urethritis, and yellow purulent secretions can appear at the urethral opening, while female infected patients often have no obvious symptoms, but they can spread from the urethra and vagina to the fallopian tubes, causing infertility. Conjunctivitis can gradually involve the whole eye, leading to blindness. After infection with gonorrhoeae, if not treated, corneal perforation may occur, leading to blindness, and fallopian tubes may also be narrowed, thickened, adhered, and blocked, which may lead to infertil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/571G01N33/531
Inventor 孙宜峰郭华燕曾冰冰
Owner ZHUHAI ENCODE MEDICAL ENG