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Blood purifying adsorbent used for removing blood toxin and preparation method

A blood purification and adsorbent technology, applied in the field of biomedicine, can solve the problems of ligand shedding, high production cost, and patient safety risks, and achieves high chemical and physical stability, stable physical and chemical properties, and good biocompatibility. sexual effect

Active Publication Date: 2015-05-13
佛山市博新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the protein A adsorbent also has some drawbacks that limit its widespread use
First of all, protein A, as a protein ligand, has high production costs and brings heavy economic burden to patients; in addition, the stability of protein ligand is not high, which brings inconvenience to the production, transportation, storage and use of adsorbents. Ligand shedding during treatment can also pose a risk to patient safety

Method used

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  • Blood purifying adsorbent used for removing blood toxin and preparation method
  • Blood purifying adsorbent used for removing blood toxin and preparation method
  • Blood purifying adsorbent used for removing blood toxin and preparation method

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preparation example Construction

[0055] Such as figure 1 As shown, the embodiment of the present invention provides a method for preparing a blood purification adsorbent for removing blood toxins, including:

[0056] S101: Activate the solid-phase carrier with an activator, the activator being epichlorohydrin, epibromohydrin or N,N'carbonyldiimidazole.

[0057] Preferably, the activator is epichlorohydrin or N,N'carbonyldiimidazole.

[0058] Preferably, the solid phase carrier is cellulose, agarose or chitosan.

[0059] S102, using 2-amino-5-methylpyridine as a ligand, and fixing it on the activated solid-phase carrier by chemical coupling to obtain a blood purification adsorbent;

[0060] Wherein, the solid-phase carrier material is in the form of porous microspheres, and its colloidal pore penetration molecular weight is 11,000-5,100,000.

[0061] Preferably, the solid-phase carrier material is in the form of porous microspheres, and its colloidal pore penetration molecular weight is 11,800-5,000,000.

[0062] Such as ...

Embodiment 1

[0137] Add 10ml of cellulose balls to the water until the total volume reaches 34ml, then add 10ml of 1mol / L sodium hydroxide solution and heat to 40 degrees. Then add 3ml of epichlorohydrin to the system and stir for two hours at 65°C for system reaction. After the reaction, rinse with a large amount of water to obtain the activated carrier. After measurement, the activation substitution degree is 1.6mmol / g.

[0138] Add 10ml of activated carrier to 150mg of 2-amino-5-methylpyridine and 40ml of 75% ethanol solution. At a constant temperature of 45 degrees, the activated carrier and 2-amino-5-methylpyridine are stirred and reacted in a 75% ethanol solution for 6 days. After the reaction, they can be washed with ethanol and water. After measurement, the coupling density is 1.2 mmol / g.

Embodiment 2

[0140] Take 10 mL of the cellulose ball, wash it with acetone to remove the water in the gel, and then disperse it in 10 mL of acetone. Weigh 2g of N,N'carbonyldiimidazole and mix with cellulose balls, and place them in a shaker at 30°C and 150rpm to react for 1 hour; after the reaction is over, wash with acetone to remove unreacted N,N'carbonyldiimidazole to obtain Activated cellulose balls. After measurement, the activation substitution degree is 2.0mmol / g.

[0141] Take 10mL of N,N'carbonyldiimidazole-activated cellulose spheres, then disperse them in 10mL of acetone, add 2g of 2-amino-5-methylpyridine to it, and place them in a shaker at 30°C and 150rpm for 3 hours. Wash with acetone and water for injection to remove unreacted substances, and obtain cellulose balls coupled with 2-amino-5-methylpyridine. After measurement, the coupling density is 1.4 mmol / g.

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Abstract

The invention discloses a blood purifying adsorbent used for removing blood toxin and a preparation method, which employs 2-amino-5-methylpyridine as a ligand, and the ligand is fixed on a solid phase carrier through chemical coupling; a solid phase carrier material employs a porous microsphere form, and the glue holes permeate the molecular weight of 11,000-5,100,000. The invention discloses a preparation method of the blood purifying adsorbent used for removing blood toxin, which comprises the following steps: activating the solid phase carrier by an activator, the activator is chloropropylene oxide, epibromohydrib or N, N' carbonyl diimidazole; employing 2-amino-5-methylpyridine as the ligand, and fixing on the activated solid phase carrier through chemical coupling to obtain the blood purifying adsorbent. By using the blood purifying adsorbent, good adsorption capability are provided on IgG, IgM type antibodies and an immunization compound and a hydrophobic molecule beta2 microglobulin. The physical and chemical properties are stable, the blood purifying adsorbent has strong acid and alkali resistant and organic solvent resistance, and the cost is low.

Description

Technical field [0001] The present invention relates to the field of biomedical technology, in particular to a blood purification adsorbent for removing blood toxins and a preparation method thereof. Background technique [0002] Autoimmune diseases are a type of disease caused by disorders of the immune system that recognize and damage normal cells, tissues, and organs. There are various autoimmune diseases, and more than 40 types have been identified. The common ones are: thyroid disease, systemic lupus erythematosus, rheumatoid arthritis, systemic sclerosis, etc. Autoimmune diseases have a very high disability rate or fatality rate, and our country is also a frequent area of ​​such diseases. According to epidemiological statistics, there are more than 1 million patients with systemic lupus erythematosus in my country, the most in the world. The incidence of rheumatoid arthritis in my country is as high as 0.35%-0.4%, and the disability rate is 15%. At present, there are no ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/22B01J20/24B01J20/28B01J20/30A61M1/38
Inventor 姜建明黄志攀
Owner 佛山市博新生物科技有限公司