Application of berberine in pharmacy

A technology of berberine and medicine, which is applied in the field of medicine preparation and can solve the problems of berberine shortage and other problems

Inactive Publication Date: 2013-04-17
SHANGHAI INST FOR ENDOCRINE & METABOLIC DISEASES
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] So far, there are not many drugs that can lower blood lipid and reduce weight when lowering blood sugar, and berberine has been reported to improve insulin resistance and lower blood su

Method used

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  • Application of berberine in pharmacy
  • Application of berberine in pharmacy
  • Application of berberine in pharmacy

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Experimental program
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Embodiment Construction

[0026] Below in conjunction with embodiment, the present invention will be further described:

[0027] The 3T3-L1 cells used in the experiment were purchased from ATCC (American Type Culture Collection); Berberine Hydrochloride (a chemical reference substance) was produced by China National Institute for the Control of Pharmaceutical and Biological Products.

[0028] 1. Cell culture and induced differentiation

[0029] The 3T3-L1 preadipocytes were inoculated on the culture plate, and the high-glucose DMEM medium containing 10% calf serum was incubated at 37°C, 5% CO 2 Culture, after 2 days of cell fusion, add 10% calf serum high glucose DMEM containing 0.5mmol / L isobutyl-methyl-xanthine, 0.25μmol / L dexamethasone and 1μg / ml insulin for 48 hours, replace Culture medium containing 1 μg / ml insulin for another 48 hours, then continue culture with 10% calf serum high-glucose DMEM, change the medium every 2 days, and 90% of 3T3-L1 cells after 7 days of induction and differentiation...

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Abstract

The invention belongs to the field of medicine preparation, and in particular relates to an application of berberine in preparing medicines for treating obesity and type-2 diabetes, medicines for suppressing fat cell differentiation and medicines for prompting phosphodiesterase activity. According to the application, a 3T3-L1 fat cell is taken as a research target, the experiment result shows that the berberine can suppress 3T3-L1 fat cell differentiation, and particularly when the berberine concentration is 10micon mol/L, the fat cell differentiation can be completely suppressed; the berberine can reduce expression of fat cell transcription factors such as C/EBPalpha, PPARgamma and the like by activating AMPK (Adenosine Monophosphate Activated Protein Kinase), and suppress the differentiation of the fat cell; and the berberine can increase the degradation of cAMP in cells and reduce the phosphorylation level of HSL (Hormone Sensitive Lipase) by reducing the suppression to PED activity so as to achieve the function of steatolysis prevention. The preparation provides experimental data and theoretic basis for treating of type-2 diabetes and obesity patients by using the berberine more reasonably in clinic.

Description

technical field [0001] The invention belongs to the field of medicine preparation, and particularly relates to the application of berberine in the preparation of medicines for treating obesity and type 2 diabetes, the preparation of medicines for inhibiting fat cell differentiation and the medicines for promoting the activity of phosphodiesterase. Background technique [0002] Obesity as a disease is seriously threatening human health. The World Health Organization Expert Committee proposed to use body mass index (BMI) as the standard for overweight or obesity, that is, BMI greater than 30kg / m 2 It is estimated that there are about 1 billion overweight or obese people in the world. In the United States, the overweight population accounts for 61% of the total population, and the obese population accounts for 26% of the total population. Obesity is not only a problem in western industrialized countries, but is also becoming prevalent in developing countries. Because obesity is...

Claims

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Application Information

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IPC IPC(8): A61K31/4375A61P3/10A61P3/04
Inventor 宁光王宁周丽斌王晓
Owner SHANGHAI INST FOR ENDOCRINE & METABOLIC DISEASES
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