Process for extracting 10-oxhydryl-2-caproleic acid from royal jelly
An extraction process and technology of royal jelly, which is applied in the field of macroporous adsorption resin adsorption chromatography to extract decenoic acid, can solve the problems of low purity of 10-HAD, unsuitable for industrial production, low recovery rate, etc., and achieves simple process flow and desorption. The effect of fast speed and high recovery rate
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Embodiment 1
[0019] (1) Ultrasonic extraction of 10-HAD with 95% ethanol: mix royal jelly and 95% ethanol at a ratio of 1:3 (m / v), oscillate and mix, place in an ultrasonic wave for ultrasonic extraction for 15 min, centrifuge at 3000r / min for 5 min, and take supernatant.
[0020] (2) Adsorption of 10-HAD by macroporous adsorption resin: Dilute the supernatant with 4 to 5 times the volume of deionized water and mix it with macroporous adsorption resin X-5 at a ratio of 1:10 (m / v), 150r / Shake and mix for 4 hours, discard the residual liquid after adsorption, collect the resin, and wash the resin 3 times with distilled water.
[0021] (3) Desorbent desorption 10-HAD: the macroporous adsorption resin X-5 after adsorption is loaded into a chromatographic column, first eluted with 3 times the column volume of 20% ethanol to remove part of the impurities, and then with a concentration of 75% ethanol Desorb 10-HDA at a flow rate of 1 mL / min and collect the desorbed solution containing 10-HAD ac...
Embodiment 2
[0024] (1) Ultrasonic extraction of 10-HAD with 95% ethanol: mix royal jelly and 95% ethanol at a ratio of 1:3 (m / v), oscillate and mix well, place in an ultrasonic wave for ultrasonic extraction for 15 min, centrifuge at 3000 r / min for 5 min, and take supernatant.
[0025] (2) Adsorption of 10-HAD by macroporous adsorption resin: Dilute the supernatant with 4 to 5 times the volume of distilled water and mix it with macroporous adsorption resin X-5 at a ratio of 1:10 (m / v), shake and mix at 150r / min Homogenize for 4 hours, discard the residual liquid after adsorption, collect the resin, and wash the resin 3 times with distilled water.
[0026] (3) Desorbent desorption 10-HAD: the macroporous adsorption resin X-5 after adsorption is loaded into the chromatographic column, and first eluted with 3 times the column volume of 20% ethanol to remove some impurities, and then with 95% ethanol Desorb 10-HDA at a flow rate of 1 mL / min and collect the desorbed solution containing 10-HAD...
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