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Process for extracting 10-oxhydryl-2-caproleic acid from royal jelly

An extraction process and technology of royal jelly, which is applied in the field of macroporous adsorption resin adsorption chromatography to extract decenoic acid, can solve the problems of low purity of 10-HAD, unsuitable for industrial production, low recovery rate, etc., and achieves simple process flow and desorption. The effect of fast speed and high recovery rate

Inactive Publication Date: 2013-04-17
SHANDONG HUAKANG HONEY PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows ultrasound waves from an alcohol solution containing ten percent or more of another chemical called acetone that breaks down into small particles when exposed to it at certain temperatures (usually between 20°C - 25°C). These tiny fragments are then collected on porous materials like activated charcoal, which have many properties such as being able to absorb water vapor effectively while still allowing oxygen molecules through freely. By exposing these fine powders onto specific areas of them they become absorbed evenly throughout their entire volume without losing any part of their structure. They also allow other compounds found naturally within foodstuffs to stick together during cooking, making them easier to remove once separated out. Overall this method provides effective ways to extract various substances including nerve agents, drugs, metabolites, proteins, carbohydrates, etc., from liquids quickly and efficiently.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding ways to produce pure 10-hydrocinnamlne-7-hexaunsymmetriproline-8 hydroxylcarboxilbdate octadecave salt called guarantidon which contains two different chemical groups: carbohemiameterotensile groupings I & II and beta lactones containing sixteen atoms. These compounds were previously identified through experiments involving their ability to stimuate cell growth, promote metabolism, suppress inflammations, resist diseases like breast disease, cervixitis, skin lesions caused by UV ray exposures, cardiac attacks, pulmonaria, gastrointestinal disorders, diarrhoea, renal calculator necrosis syndrome, brain dysfunction due to chemotherapy drugs, heart failure, stroke arteriosclerosis, menstrual bleeding, sleep apnea, rashes, dermatoses, stomach ulcers, colonies, abnormalities associated therewith immune function defenses, cognition enhancement, nutritional supplements, medicines, flavoring agents, fragrancers, taste maskants, lubricators, preservatives, heat sources, coldness liquids lycopene, laser irrigative warmers, radiatory therapy, ionizing radiophorns, ultraviolet lights, steroid elasticizers, polynaphthanes, natural rubber, plasma generators, and bioengineering techniques including gene cloning, yeast culture, solid phase separation, gel filtration, deposition mass spectetry analysis, X-ray absorbing powder detection, and others.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Ultrasonic extraction of 10-HAD with 95% ethanol: mix royal jelly and 95% ethanol at a ratio of 1:3 (m / v), oscillate and mix, place in an ultrasonic wave for ultrasonic extraction for 15 min, centrifuge at 3000r / min for 5 min, and take supernatant.

[0020] (2) Adsorption of 10-HAD by macroporous adsorption resin: Dilute the supernatant with 4 to 5 times the volume of deionized water and mix it with macroporous adsorption resin X-5 at a ratio of 1:10 (m / v), 150r / Shake and mix for 4 hours, discard the residual liquid after adsorption, collect the resin, and wash the resin 3 times with distilled water.

[0021] (3) Desorbent desorption 10-HAD: the macroporous adsorption resin X-5 after adsorption is loaded into a chromatographic column, first eluted with 3 times the column volume of 20% ethanol to remove part of the impurities, and then with a concentration of 75% ethanol Desorb 10-HDA at a flow rate of 1 mL / min and collect the desorbed solution containing 10-HAD ac...

Embodiment 2

[0024] (1) Ultrasonic extraction of 10-HAD with 95% ethanol: mix royal jelly and 95% ethanol at a ratio of 1:3 (m / v), oscillate and mix well, place in an ultrasonic wave for ultrasonic extraction for 15 min, centrifuge at 3000 r / min for 5 min, and take supernatant.

[0025] (2) Adsorption of 10-HAD by macroporous adsorption resin: Dilute the supernatant with 4 to 5 times the volume of distilled water and mix it with macroporous adsorption resin X-5 at a ratio of 1:10 (m / v), shake and mix at 150r / min Homogenize for 4 hours, discard the residual liquid after adsorption, collect the resin, and wash the resin 3 times with distilled water.

[0026] (3) Desorbent desorption 10-HAD: the macroporous adsorption resin X-5 after adsorption is loaded into the chromatographic column, and first eluted with 3 times the column volume of 20% ethanol to remove some impurities, and then with 95% ethanol Desorb 10-HDA at a flow rate of 1 mL / min and collect the desorbed solution containing 10-HAD...

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Abstract

The invention discloses a process for extracting 10-hydroxyl -2-decenoic acid (10-HAD) from royal jelly by using a macroporous adsorption resin method. The process comprises the following steps of: dissolving royal jelly in 95-percent ethanol, and performing ultrasonic extraction; adding deionized water to dilute the extracting solution, and adsorbing the 10-HAD from the extracting solution by using macroporous adsorption resin X-5; putting the resin subjected to adsorption into a chromatography column, performing desorption by using 75 to 95 percent ethanol, and collecting desorption solution containing the 10-HAD; and performing evaporation concentration and freeze drying on eluant to obtain the 10-HAD of which the purity is greater than or equal to 92.5 percent.

Description

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Claims

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Application Information

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Owner SHANDONG HUAKANG HONEY PROD
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