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Soft-rot Erwinia with plant immune activation effect, immune activation protein extracted and application thereof

A technology of Erwinia soft rot and immune activation, applied in the fields of application, plant growth regulators, plant growth regulators, etc., can solve the negative impact of chemical pesticides and other problems, and achieve the effect of improving the expression of resistance genes

Inactive Publication Date: 2013-04-17
BIOLOGY INST OF HEBEI ACAD OF SCI
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AI Technical Summary

Problems solved by technology

However, the extensive use of chemical pesticides is also showing serious negative effects

Method used

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Experimental program
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Embodiment Construction

[0015] The present invention screens and obtains a kind of Erwinia soft rot with plant immune activation Erwinia carotovora subsp. Carotovora strain Ecc 601, when this strain is used as a pathogen to infect Arabidopsis thaliana, it cannot infect Arabidopsis thaliana, and when this strain is inoculated on tobacco leaves, the tobacco leaves can be allergic and necrotic spots appear.

[0016] Erwinia soft rot Erwinia carotovora subsp. Carotovora The process of extracting EccPR protein from strain Ecc 601 is as follows:

[0017] (1) Inserting the mini-Tn5-Km transposon into Erwinia soft rot Erwinia carotovora subsp. Carotovora The mutant strain library was obtained from strain Ecc 601, and the mutant strains that could not cause allergic reactions to tobacco leaves were screened out Erwinia carotovora subsp. Carotovora strain Ecc 601108 , that is, the insertion of the transposon in this strain disrupts the Erwinia soft-rot Erwinia carotovora subsp. Carotovor...

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Abstract

The invention relates to soft-rot Erwinia with a plant immune activation effect, an immune activation protein extracted and an application thereof. The soft-rot Erwinia is preserved Chinese Common Microorganism Culture Preservation Management Center on 15th, August, 2012, with the preserving number of CGMCCNO.6433. The soft-rot Erwinia obtained in the invention can induce plants to generate resistance response so as to have the immune activation to plants. Through verification, material in the soft-rot Erwinia with the immune activation effect is a protein generated, wherein the protein is named EccPR protein for plant immune activation, so that resistance gene expression in plants is remarkably improved, thereby effectively inducing plants to have resistance effect to pathogenic bacteria.

Description

technical field [0001] The invention relates to Erwinia soft rot with plant immune activation function, immune activation protein extracted therefrom and application thereof. Background technique [0002] my country is the world's leading agricultural country, but due to various reasons, the frequency and area of ​​major diseases have increased significantly, and the resulting yield loss is about 10%. The annual loss of major crops, economic crops such as wheat, corn, cotton, and greenhouse vegetables due to pests and diseases in Hebei Province is also huge, and it is estimated that it can reach several billion yuan. In order to deal with serious crop diseases, agricultural producers use chemical pesticides extensively. The application of chemical pesticides has indeed made a great contribution to the control of crop diseases. However, the massive application of chemical pesticides is also showing serious negative effects. Therefore, the global research, development and p...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/70C07K14/27A01N63/02A01P1/00A01P21/00C12R1/18
Inventor 贾振华宋水山刘方马宏赵芊黄亚丽
Owner BIOLOGY INST OF HEBEI ACAD OF SCI