Rapid propagation method for clustered shoot through induction of aerial root of Anthurium andraeanum Lind

Abstract

The invention provides a rapid propagation method for a clustered shoot through induction of the aerial root of Anthurium andraeanum Lind. The method comprises the following steps: 1) selection of an explant; 2) induction culture of reproduction block mass; 3) induction culture of a clustered shoot; 4) propagation of the clustered shoot; and 5) acclimatization and transplant, wherein an obtained seedling is transplanted into a pot in two months so as to obtain a tissue culture seedling bred by using the method of propagation of the clustered shoot through induction of the aerial root of Anthurium andraeanum Lind. According to the invention, the method overcomes the disadvantages of a low propagation coefficient and a complex rooting culture method in induction of a clustered shoot through reproduction block mass in the prior art; hormones consisting of TDZ and NAA are utilized to induce regeneration of the clustered shoot from the aerial root of Anthurium andraeanum Lind, so a high propagation coefficient is realized, rooting and acclimatization procedures are simplified, the reproduction block mass of the aerial root has superior differentiation capability and anti-aging capability compared to calluses induced by different materials, the purposes of a fast propagation speed and conservation of production cost are achieved, and the method is applicable to large scale seedling growing in a factory.

Description

technical field [0001] The invention relates to a method for plant propagation, in particular to a method for inducing the rapid propagation of cluster buds by aerial roots of anthurium. Background technique [0002] For a long time, Anthurium has been propagated by tissue culture using leaves, petioles and stem segments as explants. Du Jingran, Chen Chunman, Zhao Bin and others have all used the above materials as explants to induce callus and cluster bud differentiation and other techniques. However, after long-term subculture, callus does not grow or does not differentiate into clump buds or only root. The research on aerial roots of Anthurium is rarely reported. Only Wang Guilan, Chen Chao, etc. have reported on the establishment of aerial root regeneration clump system of Anthurium anthurium. -D, but the proliferation coefficient of clump buds is low, and the procedure of rooting and hardening is complicated, so it is not suitable for large-scale seedling raising in fa...

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a method for rapid propagation of anthurium aerial roots to induce clustered buds, which can overcome the shortcomings of the prior art in the prior art that the regeneration mass induces clustered buds with a low multiplication coefficient and complicated rooting culture methods, and the propagation speed is fast, Save production cost and apply to large-scale seedling cultivation in factories