Anti-angiogenic compound and usage thereof
A technique for inhibiting angiogenesis and compound, which is applied in the field of medicine and achieves the effects of high safety, wide source of raw materials and low price
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Embodiment 1
[0045] Example 1 Qualitative observation of the inhibitory effect of Closantel on the generation model of zebrafish body intersegmental vessels (ISV)
[0046] Zebrafish:
[0047] The zebrafish used in this example is vascular transgenic green fluorescent zebrafish (a gene specifically expressed by zebrafish endothelial cells acts as a driver to drive the specific expression of green fluorescent protein in zebrafish vascular endothelial cells) ( figure 1 ), the feeding and use standards were strictly in accordance with the requirements of the American Institutional Animal Care and Use Committee (IACUC).
[0048] Fish water:
[0049] Configuration method: add 0.3g of sea salt (InstantOcean salts) to 1L of reverse osmosis (RO) water.
[0050] Dimethyl sulfoxide (DMSO, analytically pure):
[0051] Purchased from Aladdin (Catalog #1095515, Lot #30573). 0.1% DMSO solution (negative control) preparation: when in use, use fish culture water to prepare a working solution with a con...
Embodiment 2
[0061] Example 2 Quantitative evaluation of the inhibitory effect of Closantel on the generation model of zebrafish intersegmental vessels (ISV)
[0062] The budding of zebrafish vascular endothelial cells starts at 20hpf after fertilization, forms the main intersegmental vascular network at about 30-31hpf, such as dorsal long axis vessels (DLAV) and intersegmental vessels (ISV), and basically forms a complete body axis at 48hpf Vascular network [17] , the intact intersegmental vessels (ISVs) are clearly visible at this time. The complete intersegmental vessel mainly refers to the segment of vessel connecting the dorsal aorta (DA) and the dorsal long axis vessel (DLAV), see figure 1 (48hpf vascular transgenic fluorescent zebrafish body intersegmental vessel model). There are 28 pairs of intact intersegmental vessels (ISVs) in 48hpf zebrafish. The experimental method is as follows:
[0063] (1) Experimental grouping and embryo treatment: 300 well-developed zebrafish embryos...
Embodiment 3
[0071] Example 3 Quantitative evaluation of the inhibitory effect of Closantel on the zebrafish inferior intestinal vessel (SIV) generation model
[0072] Zebrafish subintestinal vessel (SIV, subintestinal vessel) grows on both sides of the yolk sac, and its shape is like a basket. The length of the subintestinal vessel (SIV) extending downward from the ventral side of the somites is about 50-100 μm [15-16] . See Figure 5 (72hpf vascular transgenic fluorescent zebrafish subintestinal vascular model). The experimental method is as follows:
[0073] (1) Experimental grouping and embryo treatment: 120 well-developed zebrafish embryos were taken, and the embryo development phase was 48hpf (hour-postfertilization, hpf) after fertilization, and were randomly divided into 4 groups, as shown in the following table:
[0074]
[0075] The number of zebrafish embryos in each group was 30. During the operation, the embryos were evenly distributed to 48-well cell culture plates (Gr...
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