Cell electrofusion device and method

Abstract

The invention discloses a cell electrofusion device. The cell electrofusion device is characterized in that a first rectangular groove which is sunk and used for mounting a sample carrying sheet and a second rectangular groove which is positioned in the first rectangular groove and further sunk are formed on a base; the second rectangular groove is a vacuum cavity; a joint is arranged on a side surface of the base; a flow passage allowing liquid to flow is arranged between the vacuum cavity and the joint; the joint is connected to a pressure loop; a rectangular clamping groove is formed on each of the two sides of the first rectangular groove on the base; through holes are formed on the clamping grooves; a first screw is mounted to fix a first electrode, and a second screw is mounted to fix a second electrode on the base; a square groove is formed on the sample carrying sheet, and a plurality of micropores are formed at the bottom of the groove; and the first electrode and the second electrode are clung to the bottom of the groove of the sample carrying sheet. The cell electrofusion device provides a new cell alignment method, can be fixed through cell adsorption and combined with operation of a robot, realizes pairing of a plurality of specified cells and eliminates randomness of the traditional electrofusion method.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a cell electric fusion device and a method for realizing cell electric fusion through the device. Background technique [0002] Cell fusion (cell fusion) is a cell engineering technology developed in the 1960s, also known as cell hybridization, which refers to the fusion of two or more cells by biochemical or physical methods to produce a dual parent Heterozygous cells with genetic traits, the fused cells obtain genetic material from two parental cells, have new genetic or biological characteristics, and can be used to prepare cell engineering products after cultivation, and even produce special species or strains. Cell fusion has gradually become a core technology of cell engineering. It not only provides a powerful means for the research of nucleoplasmic relationship, gene regulation and genetic complementarity, tumorigenesis, gene localization, aging control, e...

AI Technical Summary

Problems solved by technology

Microfluidic chips are portable and easy to integrate, but the production cost of microelectrodes, especially high-density microelectrode arrays, is high, the requirements for electric field control are high, and the accuracy of cell pairing is also limited.

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