A high-efficiency degradation bacterial agent for recycled papermaking wastewater and its preparation method
A high-efficiency technology for papermaking wastewater, which is applied in the field of high-efficiency degradation bacterial agents for recycled papermaking wastewater and its preparation, can solve the problems of long bacterial micelles or biofilm cycles, bacteria that have not been artificially domesticated, and cannot achieve degradation effects, etc., to achieve Easy delivery, improved effect, and strong adaptability
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Embodiment 1
[0029] Example 1 Preparation method of recycled papermaking wastewater efficiently degrading composite bacterial agent
[0030] S1 strain isolation and screening
[0031] Collect the wastewater from the wastewater treatment tank of the paper mill, add it to three kinds of liquid selective medium, and shake it with a shaker flask for culture, the shaker speed is 200r / min, the culture temperature is 36±1°C, and the culture time is 48h. The cultured bacterial suspension was diluted by the ten-fold serial dilution method, and the last few dilutions were selected and transferred to the solid selective culture plate by the coating method, cultured at 36±1°C for 48 hours, and the single colony on the plate was picked to proliferate After the cultivation, the waste water organic matter degradation experiment was done, and the strain with the highest COD degradation rate was selected as the starting bacteria for the production of the compound bacterial agent.
[0032] Production of S2...
Embodiment 2
[0034] The use of embodiment 2 composite bacterial agents
[0035] It can be added at the initial stage of the establishment of the biological pool, and the compound bacterial agent produced in Example 1 can be directly added in an amount of 0.1g / L, and it can cooperate with the natural bacteria to accelerate the formation of bacterial micelles or biofilms; it can also be run in the biological pool During the process, when the influent water quality fluctuates or is polluted by toxic and harmful substances, when the natural bacteria die more, it will be added, and the added amount is 0.01g / L.
Embodiment 3
[0036] The contrast of embodiment 3 effects
[0037] Through the use of this patent, the domestication period of the biological pool can be shortened from 10 days to several months to 5 to 20 days, and it can be restored by adding bacterial agents when the biological pool encounters external unfavorable factors that cause fluctuations in the microbial system. Normal function of the biological treatment tank. The waste water degraded by this patent can reduce COD from 600mg / L to less than 100mg / L, meeting the discharge standard.
[0038] Prepare 5 biological pools, when the initial COD values are the same, add the compound bacterial agent obtained in Example 1 of the application according to the method of Example 2, one biochemical pool does not add the complex bacterial agent, and the remaining 3 biochemical pools are added separately Add Bacillus megaterium, Pseudomonas stutzeri and Pseudomonas alcaligenes, observe for 20 days, and test 5 biochemical pools respectively at ...
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