Dendrobe aqueous extract and application thereof

A technology of water extract and dendrobium, which is applied to the water extract of dendrobium and its application field, can solve problems such as no research report on dendrobium and its preparations, and achieve the effects of strong pharmacological action and shortening bleeding time.

Inactive Publication Date: 2013-05-15
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] But so far, there is no research report on t...

Method used

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  • Dendrobe aqueous extract and application thereof
  • Dendrobe aqueous extract and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1: the preparation of dendrobium water extract

[0033] Dendrobium water extract of the present invention, obtain through following method:

[0034] a. Cut the dendrobium into pieces with scissors and add it to water for boiling according to the ratio of W / V 1:10-20. After the dendrobium is boiled in water, keep it for 2-4 hours, then filter and discard the residue with gauze, and collect the dendrobium for extraction. liquid;

[0035] b. Repeat step a for 2 to 4 times, combine the extracts of Dendrobium each time, and obtain the powdered Dendrobium aqueous extract after the extracts are conventionally freeze-dried.

[0036] Described dendrobium refers to the wild or cultivated fresh dendrobium or dried dendrobium purchased in the market.

Embodiment 2

[0037] Embodiment 2: the hemostatic effect of dendrobium aqueous extract

[0038] The water extract used in this example is the Dendrobium water extract prepared in Example 1.

[0039] Select C57BL / 6 strain mice, body weight 20-25g, male. They were randomly divided into three groups, namely the control group (0 g / kg), the low-dose Dendrobium water extract group (0.5 g / kg), and the high-dose Dendrobium water extract group (1.0 g / kg), with 9 rats in each group. The bleeding time of the tail vein of the mice was measured 0.5 h after administration. The method of administration was that the control group was gavaged with tap water, and the Dendrobium water extract low and high dose groups were gavaged with different doses of Dendrobium water extract solutions. Dendrobium water extract solution is prepared by using the Dendrobium water extract prepared in Example 1. During the experiment, the mouse was put into a special holder, and then the tip of the tail of the mouse was tran...

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Abstract

The invention relates to dendrobe aqueous extract and application thereof, and belongs to the technical field of pharmacy. The dendrobe aqueous extract disclosed by the invention is obtained by the following method: a, cutting dendrobe into pieces by scissors and adding the dendrobe pieces to water in the proportion W/V of 1:(10-20) for boiling; continuously keeping the boiling state for 2 hours to 4 hours after the drendrobe is boiled in the water; removing the residues by filtering through gauze and collecting the water extract; b, repeating the step a for twice to four times, combining the extracting solution every time; and freezing and drying the extracting solutions to obtain powder dendrobe water extract. The dendrobe is wild or artificially-cultivated fresh dendrobe or dried dendrobe. The invention further relates to the application of the dendrobe aqueous extract in preparing hemostatics. According to the dendrobe aqueous extract and the application thereof disclosed by the invention, the dendrobe aqueous extract provided by the invention has strong pharmacological action, the bleeding time is remarkably shortened and the medical prospect of the dendrobe aqueous extract in developing and preparing hemostatics are disclosed; and secondly, a novel application field of the dendrobe is developed.

Description

technical field [0001] The invention relates to a dendrobium dendrobium water extract and its application, belonging to the technical field of pharmacy. Background technique [0002] Blood is an important substance for sustaining life. It contains various nutrients, such as inorganic salts, oxygen, and cell metabolites, hormones, enzymes, and antibodies. It has the functions of regulating organ activities and defending against harmful substances. The blood of an adult accounts for about 8% of its own weight, and there is about 60-80 ml of blood per kilogram of body weight. Slow and small amounts of bleeding in the human body can achieve hemostasis through mechanisms such as vasoconstriction and platelet aggregation to form blood clots. However, when the bleeding in the human body reaches more than 20% of the total blood volume, serious symptoms such as blood pressure drop and shock will occur. If the bleeding exceeds 40%-50% of the total blood volume, life-threatening will ...

Claims

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Application Information

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IPC IPC(8): A61K36/8984A61P7/04
Inventor 田洋盛军白忠彬郝淑美高晓余史崇颖方崇业
Owner YUNNAN AGRICULTURAL UNIVERSITY
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