Method for producing high-concentration lactic acid by lignocellulose at high temperature

A lignocellulose, high-concentration technology, which is applied in the field of efficient bioconversion of lignocellulose to produce high-concentration and high-optical purity lactic acid, can solve the problems of limiting the production rate and yield of lactic acid, low cellulose enzymatic hydrolysis efficiency, etc. The risk of bacterial contamination, the effect of improving the efficiency of enzymatic hydrolysis and reducing the dosage

Inactive Publication Date: 2013-05-15
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology

Therefore, in the conventional research on the simultaneous saccharification and fermentation of lignocellulosic raw materials to produce lactic acid, the temperatu

Method used

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  • Method for producing high-concentration lactic acid by lignocellulose at high temperature
  • Method for producing high-concentration lactic acid by lignocellulose at high temperature

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Embodiment 1

[0043] see attached figure 1 , the dried corn stalks were mixed with dilute sulfuric acid solution until the water content was 33% (w / w), the sulfuric acid concentration was 2.5% (w / w), and pretreated at 190°C for 3 minutes; the pH of the pretreated material was adjusted to 5.5, and the water content was 60 % (w / w), add Amorphotheca resinae ZN1, the strain used for biological detoxification, and culture in solid state at 25°C for 7 days; harvest the material, sterilize the material at 115°C for 20 minutes, add it to the fermenter after cooling, and adjust the solid in the fermenter The content is 25% (w / w), the dosage of cellulase is 15FPU / gDM, pre-saccharification at 50°C for 8h, the pH of the fermentation process is controlled by 5M sodium hydroxide at 5.5, fermentation is at 48°C for 72 hours, and the final lactic acid concentration is 81.80g / L .

Embodiment 2

[0045] see attached figure 2 , the dried corn stalks were mixed with dilute sulfuric acid solution until the water content was 33% (w / w), the sulfuric acid concentration was 2.5% (w / w), pretreated at 190°C for 3 minutes, the pH of the pretreated material was adjusted to 5.5, and the water content was 60 %(w / w), add the strain Amorphotheca resinae ZN1 used for biological detoxification, and culture in solid state at 25°C for 7 days; harvest the material, sterilize the material at 115°C for 20 minutes, add it to the fermenter after cooling, and the solid content in the fermenter The mass fraction is 30%, the dosage of cellulase is 15FPU / gDM, pre-saccharification at 50°C for 8h, the pH of the fermentation process is controlled at 5.5 by 5M sodium hydroxide, fermentation is at 48°C for 72 hours, and the final lactic acid concentration is 101.60g / L.

[0046] The lignocellulose raw material involved in the present invention is not limited to corn stalks, and can also be one of whea...

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Abstract

The invention relates to a method for producing high-concentration lactic acid by lignocellulose at a high temperature. The method comprises the following steps of 1, carrying out solid-state biological detoxification of a lignocellulose raw material under the condition of a very low water use amount, 2, culturing pediococcusacidilactici DQ2 by a liquid medium, 3, carrying out simultaneous saccharification and fermentation production of lactic acid in a fermentation tank, and 4, adding the fermented culture and the lignocellulose raw material obtained by the step 2 and 3 into a reactor for simultaneous saccharification and fermentation, adjusting required solid content, adding a nutritive salt and cellulase into the reactor, and carrying out simultaneous saccharification and fermentation of the lignocellulose raw material under the conditions of a temperature of 48 DEG C and a pH value of 5.5 to obtain high-concentration lactic acid. Lactic acid is produced by lignocellulose as a raw material at a temperature of 48 DEG C so that cellulose enzymolysis efficiency is improved; a cellulase use amount is reduced; a cooling water use amount is reduced; a production cost is reduced; and a risk of microbiological contamination in fermentation is reduced.

Description

【Technical field】 [0001] The invention relates to the field of biomanufacturing and biorefining of bio-based chemicals, specifically, using lignocellulose such as corn stalks as raw materials to efficiently biotransform lignocellulose under high temperature conditions to produce lactic acid with high concentration and high optical purity method. 【Background technique】 [0002] Lactic acid is an important intermediate in the synthesis of various chemicals and can be widely used in food, medicine, cosmetics, leather, textiles, environmental protection and agricultural production. The most important use at present is to produce degradable polylactic acid products (Polymeric lactic acid, PLA) from L-lactic acid that can replace polymeric materials such as polyethylene and polypropylene. This degradable polymer product has been industrialized in large quantities and is the most important biomaterial to replace petroleum-based polymer materials. With the wide application of poly...

Claims

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Application Information

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IPC IPC(8): C12P7/56C12P19/14C12R1/01C12R1/225
Inventor 鲍杰赵凯楚德强张建
Owner EAST CHINA UNIV OF SCI & TECH
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