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Application of canarium album extract in preparation of medicaments, foods or daily necessities for preventing and treating decayed teeth

The technology of green olive and extract is applied to the application field of green olive extract in the preparation of medicines, food or daily necessities for preventing and treating dental caries, which can solve the problems such as the inhibition of oral anaerobic bacteria by the green olive extract, and achieve convenient prevention and treatment. Effect

Active Publication Date: 2015-01-07
LOGISTICS UNIV OF CAPF
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

But so far there is no report on the inhibition of oral anaerobic bacteria by green olive extract

Method used

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  • Application of canarium album extract in preparation of medicaments, foods or daily necessities for preventing and treating decayed teeth
  • Application of canarium album extract in preparation of medicaments, foods or daily necessities for preventing and treating decayed teeth
  • Application of canarium album extract in preparation of medicaments, foods or daily necessities for preventing and treating decayed teeth

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Experimental program
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preparation example Construction

[0030] The preparation steps of the green olive extract are: taking green olive branches, leaves or fruits as raw materials, refluxing extraction with water or an aqueous ethanol solution, filtering, and concentrating the filtrate under reduced pressure to obtain a concentrate, and drying to obtain the green olive extract.

[0031] The preparation parameters of the green olive extracts in Examples 1-14 are shown in Table 1.

[0032] Table 1

[0033]

Embodiment 15

[0034] Example 15 Green olive extracts in vitro inhibition of caries-inducing Streptococcus mutans, Streptococcus sobrinus and Lactobacillus test

[0035] Streptococcus mutans CGMCC12500, purchased from China Common Microbial Species Collection and Management Center.

[0036] Streptococcus sobrinus ATCC27351 was purchased from Beijing Beina Chuanglian Institute of Biotechnology.

[0037] Lactobacillus sp. ACCC11026 was purchased from Beijing Beina Chuanglian Institute of Biotechnology.

[0038] Test method: Dissolve plant extracts in TPY liquid medium by dilution method, prepare different concentrations of medicinal solutions, and set up blank control tubes (only medicinal liquid and medium) and unmedicated control tubes (add only Bacteria and culture medium) each. Pipette 50μL of bacterial suspension into the experimental tube and the non-medicated control tube, place in an anaerobic incubator, culture for 48h under 80%N2, 10%CO2, 10%H2, and 37℃, compare different concentrations of ...

Embodiment 16

[0042] Example 16 The effect of green olive extract on the adhesion of Streptococcus mutans was determined.

[0043] Test method: Inoculate the Streptococcus mutans strains recovered for 48 hours in TPY liquid medium, culture anaerobic at 80% N2, 10% CO2, 10% H2, and 37°C for 18 hours, and adjust after smear inspection is pure culture The final concentration of the bacterial suspension reaches 5×107CFU / mL. Mix 1 part of the bacterial suspension with 10 parts of TPY liquid medium containing green olive extract and inoculate it in a petri dish. Each sample experiment group has 3 parallel petri dishes. . 80%N2, 10%CO2, 10%H2, 37℃, anaerobic culture for 48h, discard the supernatant after culture, then quantify the TPY liquid medium, pipetting to resuspend the bacteria attached to the glass surface of the petri dish TPY medium was used as a blank, and the turbidimetric method was used to determine the adhesion of bacteria and the results of drug action. The experimental results are ...

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Abstract

The invention discloses an application of a canarium album extract in preparation of medicaments, foods or daily necessities for preventing and treating decayed teeth. Experiments prove that the canarium album extract has an obvious function of inhibiting streptococcus mutans, streptococcus sobrinus and lactobacillus inducing the decayed teeth, can inhibit the adhesivity of the streptococcus mutans, streptococcus sobrinus and lactobacillus, and has a function of inhibiting the acidogenicity of the streptococcus mutans, streptococcus sobrinus and lactobacillus, so that the canarium album extract is very suitable for preventing and treating the decayed teeth. The canarium album extract is particularly suitable to be prepared into various medicament dosage forms or various foods and daily necessities, and is convenient to prevent and treat the decayed teeth.

Description

Technical field [0001] The invention relates to the application of a green olive extract in the preparation of medicines, foods or daily necessities for preventing and curing dental caries. Background technique [0002] The plant extracts involved in the present invention come from the plant extracts of green olives which are abundant in my country. Green olive tree Canarium album (Lour.) Raeusch. is an economic fruit tree planted in parts of southern my country. Its mature and dry fruit is ovoid and blue in color. It is also known as green fruit, green seed, white olive, green olive, etc. The effective active ingredient is olive polyphenols, which has the functions of clearing heat, improving throat, promoting body fluid, detoxification, immune regulation, lowering blood pressure, lowering blood lipids, and removing free radicals. It is used to treat sore throat, cough, polydipsia, fish and crab poisoning Wait [1,2 ], its output is huge and its nutritional value is rich. Green o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/32A61K8/97A61K9/12A61P1/02A23L1/29A61Q11/00A61K131/00A23L33/00
Inventor 刘岱琳延慧君陈虹董晓青马卫俊赵艳敏高颖
Owner LOGISTICS UNIV OF CAPF
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