Beta-glucosaccharase gene for coding glycosyl hydrolase family 1 and application thereof

A technology of glycosyl hydrolase and glucosidase, which can be used in application, genetic engineering, plant genetic improvement and other directions, and can solve the problems of low cellulase activity, limited application scope, and impact on enzymatic hydrolysis efficiency.

Active Publication Date: 2014-06-11
GUANGXI UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, there are a variety of mycotoxins in the fermentation products of Trichoderma and the obtained cellulase activity is low, especially the activity of β-glucosidase is very low, resulting in the accumulation of cellobiose in the reaction system and affecting the enzymatic hydrolysis efficiency, so its application limited range

Method used

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  • Beta-glucosaccharase gene for coding glycosyl hydrolase family 1 and application thereof
  • Beta-glucosaccharase gene for coding glycosyl hydrolase family 1 and application thereof
  • Beta-glucosaccharase gene for coding glycosyl hydrolase family 1 and application thereof

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Embodiment Construction

[0020] The following implementation methods are for better explaining the present invention, but should not be construed as limiting the present invention.

[0021] The materials used in the examples of the present invention include: Escherichia coli (Escherichia coli) strain XL1-blue was purchased from Dalian TaKaRa Company; expression vector pSE380 was purchased from Stratagene Company; TaKaRa, MBI.

[0022] The present invention will be described in detail below by embodiment:

[0023] 1) Extraction of Streptomyces sp.GX6 genomic DNA

[0024] Genomic DNA of Streptomyces sp.GX6 was extracted according to the instruction of BioFlux company's Bacterial Genomic DNA Extraction Kit Biospin Bacteria Genomic DNA Extraction Kit (Cat. No. BSC12S1).

[0025] The extracted genomic DNA of Streptomyces sp.GX6 was sent to Huada Gene Company for genome sequence determination.

[0026] 2) Analysis of the gene sequence encoding β-glucosidase in the genome sequence of Streptomyces sp.GX6 ...

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Abstract

The invention provides a beta-glucosaccharase gene for coding a glycosyl hydrolase family 1 and an application thereof. The nucleotide sequence of the beta-glucosaccharase gene is shown in SEQ ID NO:1, the nucleotide sequence of beta-glucosaccharase S-bgl4 coded by the gene is shown in SEQ ID NO:2, and the beta-glucosaccharase S-bgl4 can be applied to decomposition of cellobiose.

Description

technical field [0001] The present invention relates to a β-glucosidase gene encoding glycosyl hydrolase family 1 and its application. The protein encoded by the gene can be used to degrade cellobiose. Background technique [0002] β-glucosidase is a kind of cellulase system, which catalyzes the hydrolysis of β-D-glucosidic bonds, and has important value in the carbohydrate metabolism of humans, animals, plants and microorganisms (Ramakrishnan Anish, Mohammad Safikur Rahman, and Mala Rao, 2007, Application of cellulase from an alkalothermophilic Thermomonospora sp. in biopolishing of denims, Biotechnol. Bioeng., 96(1):48-56.). According to the classification of amino acid sequence, people divide β-glucosidase into glycoside hydrolase family 1 and 3. The β-glucosidase in family 1 mainly comes from bacteria and plants; the β-glucosidase in family 3 mainly comes from fungi, bacteria and plants. In addition to glucosidase activity, the β-glucosidase in family 1 also has strong...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/56C12N9/42C12N15/63C12P19/14
Inventor 杜丽琴王子龙韦宇拓庞浩黄日波谭慧敏闭海
Owner GUANGXI UNIV
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