Preparation method of CuS nanoparticles, product and application thereof
A nanoparticle and solution technology, which is applied in the field of preparation of copper sulfide nanoparticles, can solve problems such as variability and instability of biomolecules, and achieve the effects of low biotoxicity, good biocompatibility and dispersibility, and mild preparation conditions
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[0034] The preparation method of the present invention may also include a purification step S3: for example, dialysis may be used for purification operation, at room temperature, sequentially dialyze the solution of sulfhydryl-modified CuS nanoparticles using alkaline buffer solution and redistilled water to remove small inorganic molecules. The alkaline buffer of this step can use phosphate buffered saline (PBS), especially the phosphate buffered saline that pH is 9, or other alkaline buffers.
[0035] The solution of the finally obtained sulfhydryl-modified CuS nanoparticles can be stored lyophilized at 4° C., so that it can be stored for a longer period of time.
[0036] The preparation method of the invention is carried out at room temperature, and the reaction conditions are mild; the operation is simple and the reproducibility is good; the biomolecular synthesis is adopted, and the cytotoxicity of the nanoparticles can be reduced.
[0037] The thus prepared sulfhydryl-mo...
Embodiment 1
[0043] Add 3mL of 0.1mol / L glutathione solution to 1.5ml of 0.1mol / L copper chloride solution, mix well, and add 25ml of water.
[0044] Use 1mol / L NaOH solution to adjust the pH to 9, add 1.5mL 0.1mol / L thioacetamide, and shake gently at room temperature for 12h.
[0045] Separation of small inorganic molecules by dialysis: transfer the final product of the reaction into a dialysis bag. At room temperature, dialyze in 500ml PBS buffer solution (pH 9) for 24 hours, change the medium once every 12 hours, and then dialyze in 500ml double distilled water for 12 hours.
[0046] The dialysate was lyophilized and stored at 4°C.
Embodiment 2
[0048] Add 0.5mL 0.1mol / L glutathione solution to 3ml 0.1mol / L copper sulfate solution, mix well, and add 25ml water.
[0049] Use 1mol / L NaOH solution to adjust the pH to 9, add 1.5mL 0.1mol / L thioacetamide, and shake gently at room temperature for 12h.
[0050] Separation of small inorganic molecules by dialysis: transfer the final product of the reaction into a dialysis bag. At room temperature, dialyze in 500ml PBS buffer (pH 9) for 72 hours, change the medium once every 12 hours, and then dialyze in 500ml double distilled water for 6 hours.
[0051] The dialysate was lyophilized and stored at 4°C.
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