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Protein composition Cry6A/Cry55A having insecticidal synergistic effect for meloidogyne incognita, and application

A southern root-knot nematode, synergistic effect technology, applied in the application, biocide, nematicide and other directions, can solve the problems of lack of obvious synergistic effect and lack of obvious synergistic effect of Spodoptera exigua

Active Publication Date: 2013-06-05
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The experimental results of Cai Qiliang et al. (2003) showed that the insecticidal protein Vip83 and Cry1Ac10 and Vip83 and Cry1Ca had antagonistic effects on cotton bollworm, but the synergistic effect on beet armyworm (Spodotera exigua) was not obvious; The synergistic effect of diamondback moth (Plutela xylostella) is not obvious, but Vip83 and Cry1Ca have synergistic effect on diamondback moth
However, there is no report on the activity of these insecticidal crystal proteins against M. incognita, especially the control of M. research has not yet seen any reports

Method used

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  • Protein composition Cry6A/Cry55A having insecticidal synergistic effect for meloidogyne incognita, and application
  • Protein composition Cry6A/Cry55A having insecticidal synergistic effect for meloidogyne incognita, and application
  • Protein composition Cry6A/Cry55A having insecticidal synergistic effect for meloidogyne incognita, and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Crystal proteins Cry5B, Cry6A and Cry55A have high activity against Meloidogyne incognita

[0026] 1. Purification and quantitative analysis of crystal proteins Cry5B, Cry6A and Cry55A

[0027] Bacillus thuringiensis strains BMB0250, BMB0215 and BMB0224 (see literature for the above three strains: Suxia Guo, Mei Liu, Donghai Peng, Sisi Ji, Pengxia Wang, Ziniu Yu, and Ming Sun. New Strategy for Isolating Novel Nematicidal Crystal Protein Genes from Bacillus thuringiensis Strain YBT-1518. Applied and Environmental Microbiology, 2008, 6997-700.) was constructed in the previous research of the inventor's research group and can express crystal proteins Cry6Aa (long rice-like crystals, 54KDa), Cry5Ba (rhombic crystals, 140KDa) and Recombinant bacteria of Cry55Aa (long rice-like crystals, 45KDa) (Guo et al., 2008). In order to conduct subsequent bioassays and complex activity studies, the applicant used BMB0250 (i.e. the recombinant strain of Cry6Aa (Guo et al., 20...

Embodiment 2

[0044] Example 2: Determination of the activity of crystal proteins Cry6A, Cry5B and Cry55Aa in pairs with different ratios against Meloidogyne incognita

[0045] 1. Calculation method for the insecticidal synergistic effect of the nematode crystal protein composition on root-knot nematode incognita

[0046] The synergy coefficient (SF) calculation formula proposed by Tabashnik et al. (1992) was used to evaluate the insecticidal synergistic effect. The specific formula is as follows:

[0047] 1 LC 50 ( m ) = Ra LC 50 ( a ) + R b LC 50 ...

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Abstract

The invention belongs to the technical field of insecticidal microbes, and discloses a nematicidal crystal protein composition having an insecticidal synergistic effect for meloidogyne incognita. The composition is obtained by combining nematicidal crystal protein Cry6A and Cry55A from bacillus thuringiensis according to different proportions. The composition has significant insecticidal synergistic effect for the meloidogyne incognita within a mass ratio of Cry6A to Cry55A ranging from 1:5-5:1. When the mass ratio of Cry6A to Cry55A is 1 : 1, the composition has the highest insecticidal synergistic effect for the meloidogyne incognita. The protein composition disclosed by the invention has very large application potential in the aspect of biological prevention and control for the plant root-knot nematode.

Description

technical field [0001] The invention relates to a group of insecticidal crystal protein compositions derived from Bacillus thuringiensis, which have obvious insecticidal synergistic effect on root-knot nematode (Meloidogyne incognita), and can be used to prevent and control pests that are extremely harmful to agricultural production. Meloidogyne incognita. Background technique [0002] Plant pathogenic nematodes are an important class of pathogenic organisms, many of which are internationally recognized as destructive pests. It is estimated that plant pathogenic nematodes cause an annual loss rate of 12.3% of major crops in the world, and the annual direct economic loss exceeds 100 billion US dollars, accounting for almost half of the losses caused by agricultural pests (Chitwood, 2003). Among them, the most harmful ones are plant root-knot nematodes and cyst nematodes, and the most harmful root-knot nematodes are southern root-knot nematode (Meloidogyne incognita), peanut ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N47/44A01P5/00
Inventor 孙明彭东海阮丽芳柴陆军鞠守勇王奋山
Owner HUAZHONG AGRI UNIV