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A kind of classification method of purple sweet potato anthocyanins

A grading method, anthocyanin technology, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., to achieve low cost and good industrialization prospects

Active Publication Date: 2017-10-27
JIANGSU ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

The present invention mainly aims at the technical problem that purple sweet potato anthocyanins with different degrees of acylation cannot be separated efficiently in large quantities. Through a simple separation method of multiple column chromatography, the preliminary purified purple sweet potato anthocyanins are classified according to different degrees of acylation. Separately obtain acyl anthocyanin, monoacyl anthocyanin and diacyl anthocyanin fractions

Method used

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  • A kind of classification method of purple sweet potato anthocyanins
  • A kind of classification method of purple sweet potato anthocyanins
  • A kind of classification method of purple sweet potato anthocyanins

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Embodiment

[0015] Wash 1 kg of purple sweet potato and cut it into 1 cm small pieces, add 2 liters of 95% edible alcohol, perform high-speed beating, keep warm at 60°C for 1 hour, cool, and centrifuge. The filtrate is concentrated in vacuum to about 300 milliliters, which is the crude extract, and the crude extract is put on a 500 mm × 50 mm macroporous resin column, the resin model is AB-8, and the loading flow rate is 300 ml / hour, and then the column is flushed with deionized water. After the effluent was clarified, the resin column was eluted with 750 ml of 70% aqueous ethanol as the eluent at a flow rate of 300 ml / hour. The eluent was concentrated in vacuo to 150 ml, 300 ml of ethyl acetate was added to extract fat-soluble impurities, and the aqueous phase was collected as purified purple sweet potato anthocyanins. Immediately put 150 ml of the extracted water phase on a 500 mm×50 mm polyamide resin column, the flow rate of the sample is 300 ml / hour, then flush the column with 500 ml...

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Abstract

The invention provides a method for grading purple potato anthocyanins, which belongs to the technical field of deep processing of agricultural products. Including extraction of purple sweet potato anthocyanins with edible alcohol, preliminary purification of the crude extract on AB‑8 macroporous resin column, extraction of fat-soluble impurities with ethyl acetate, immediate application of polyamide resin column, and deionized water, 30% ethanol aqueous solution and 70% ethanol aqueous solution are used as eluents to elute the resin column to obtain three anthocyanin components. Through HPLC-MS detection, the three components were acyl purple potato anthocyanins, monoacyl purple potato anthocyanins and diacyl purple potato anthocyanins. This method is low in cost, high in efficiency, safe and reliable, suitable for mass production, and has a good industrialization prospect. The obtained anthocyanin products with different degrees of acylation can be used as good materials for theoretical research on anthocyanins, and can also be used as natural food pigments, which can be used in different fields according to different stability requirements.

Description

1. Technical field [0001] The invention relates to a method for grading purple potato anthocyanins, which belongs to the technical field of deep processing of agricultural products. 2. Background technology [0002] Research on purple sweet potato at home and abroad shows that purple sweet potato is rich in anthocyanins, which has strong physiological activities and functions such as lowering blood pressure, lowering blood fat, anti-mutation, anti-oxidation, and anti-tumor. At present, more than 20 kinds of anthocyanins have been identified from purple sweet potato, including acyl anthocyanins, monoacyl anthocyanins and diacyl anthocyanins, and most of the anthocyanins in purple sweet potato are acyl anthocyanins. As a safe and non-toxic natural food coloring, anthocyanins have great development potential in food, health care products, medicine, cosmetics, etc., but natural pigments are easily affected by various conditions and become unstable, which limits Its wide applica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/08C07H17/065
Inventor 闫征李春阳黄午阳王兴娜王帆
Owner JIANGSU ACAD OF AGRI SCI
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