Fermentation culture method for bacillus subtilis PTS-394

A technology of PTS-394 and Bacillus subtilis, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problem of low bacterial content and achieve the effect of increasing bacterial content

Active Publication Date: 2013-06-12
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The object of the present invention is to: aim at the actual problem that the bacterium content in the present Bacillus subtilis PTS-394 living bacteria preparation is lower, provide a kind of m

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fermentation culture method for bacillus subtilis PTS-394
  • Fermentation culture method for bacillus subtilis PTS-394
  • Fermentation culture method for bacillus subtilis PTS-394

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Determine the carbon source and nitrogen source of the culture medium

[0014] The basic fermentation medium of Bacillus subtilis PTS-394 (disclosed in Example 3 in the patent application with publication number CN102550607A): 20 g corn flour, 10 g soybean meal, 5 g soybean meal, 1.5 g yeast powder, CaCO 3 3 g, NaCl 0.5 g, fish meal 10 g, make up to 1000 mL, pH 7.5.

[0015] Replace the 20 g / L corn flour in the above basic fermentation medium with sucrose, soluble starch, corn starch, maltose, lactose, and xylose in the same amount (20 g / L) of 6 different carbon sources to form different carbon sources. base. Replace the 10 g / L soybean meal powder of the basic fermentation medium with soybean meal, tryptone, yeast extract powder, beef extract, peptone, and fish meal in equal amounts (10 g / L) of 6 different nitrogen sources, and mix them into different nitrogen sources. Medium.

[0016] Adjust the pH of the culture media with different carbon and nitrogen sources t...

Embodiment 2

[0021] Example 2 Determine the key factors of fermentation medium composition

[0022] Using Plackett-Burman experimental design, from Example 1 Bacillus subtilis PTS-394 medium components (corn starch, soybean powder, yeast powder, CaCO 3 , NaCl, fish meal) key factors were screened out of 6 factors. The PB experiment design is shown in Table 2:

[0023] Table 2 Plackett-Burman experimental design and results of medium component factors

[0024]

[0025] Note: In the experiment, each factor takes two levels. The high level "1" means that the content of the component is 1.5 times that of the initial fermentation medium; the low level "-1" means that the content of the component is 0.5 times that of the initial fermentation medium.

[0026] Regression analysis is performed on the data in Table 2, and the main factor coding equation is:

[0027] Y 0 =9.47+0.12A+0.055B-0.032C+0.020D+9.095E-003E-0.012F

[0028] Where: Y 0 Is the predicted value of bacteria content (cfu / mL) (the same below...

Embodiment 3

[0032] Example 3 Optimal ratio of key factors of fermentation medium

[0033] Analyzing the results of the PB experiment, we can see that the three factors of corn starch, soybean meal and yeast powder are the key factors affecting the content of Bacillus subtilis PTS-394, according to the fitting equation Y 0 Each variable coefficient determines the climbing direction and step length of the key factors, and the steepest climbing experiment is carried out. The climbing experiment design and results are shown in Table 4:

[0034] Table 4 The steepest climbing experiment design and results of the key factors of the medium composition

[0035]

[0036] It can be seen from Table 4 that among the combination of 3 factors, the number 4 combination has the largest bacterial content, so this combination is selected as the central value of the response surface experiment, and the next RSM experiment design is carried out.

[0037] According to the results of the steepest climbing experiment (T...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a fermentation culture method for bacillus subtilis PTS-394. The fermentation culture method comprises the following steps of: inoculating a bacillus subtilis PTS-394 seed solution in a fermentation culture medium and carrying out fermentation culture for two days on a shaking table. The fermentation culture method is characterized in that a carbon source in the fermentation culture medium is corn starch, and a nitrogen source is soybean powder; three key factors affecting bacterial content predicated value are corn starch, soybean powder and yeast powder; the content of the three key factors in the fermentation culture medium is 11.48g/L, 13.35g/L and 0.90g/L, respectively; three key factors affecting the bacterial content and the inhibition zone diameter in the fermentation culture conditions are temperature, an initial pH value and liquid filling amount; and in the fermentation culture process, the temperature is 30.84 DEG C, the initial pH value is 6.93 and the liquid filling amount is 65.15mL/250mL.

Description

technical field [0001] The invention relates to a method for fermenting and cultivating Bacillus subtilis PTS-394, in particular to a method for cultivating Bacillus subtilis PTS-394 after optimizing the fermentation medium and culture conditions by using the Plackett-Burman method and the response surface method. Background technique [0002] With the adjustment of the planting industry structure, the planting area of ​​protected vegetables is increasing year by year. The high-yield and continuous cropping of protected vegetable planting layout provides sufficient nutrition and breeding conditions for pests and diseases. In recent years, there have been frequent outbreaks of soil-borne diseases (bacterial wilt, blight, root-knot nematode, etc.) of solanaceous vegetables in facilities. Bacterial wilt is a destructive soil-borne bacterial disease. Although the aboveground part of the disease appears to be drooping at the beginning of the disease, the leaves remain green, henc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12R1/125
Inventor 刘邮洲乔俊卿陈志谊王治林
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap