A Fluorescence Method for Rapid Determination of Fatty Acid Content
A technique for rapid determination of fatty acids, used in fluorescence/phosphorescence, material excitation analysis, etc.
Active Publication Date: 2016-01-13
JIANGNAN UNIV
View PDF5 Cites 1 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, there is no report on the use of fluorescence spectroscopy to determine the composition of fatty acids in edible oils.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0029] Experimental steps:
[0030] (1) Sampling: place a solid sample between two quartz plates, or place a liquid sample in a quartz cuvette;
[0031] (2) Fluorescence measurement of the sample: Scan the sample for fluorescence and collect its two-dimensional fluorescence spectrum;
[0032] (3) Model establishment: Chemometrics method was used to construct the regression model of fluorescence spectral data and fatty acid content of oil;
[0033] (4) Validation of the model: use the method of internal cross-validation to conduct correlation analysis and significance analysis between the measured value and the predicted value to verify the model;
[0034] (5) Determination of fatty acid content: Take the sample to be tested according to the method in step (1), measure the fluorescence of the sample according to the method in step (2), and substitute the fluorescence data into the regression model to obtain the fatty acid content.
[0035] The solid sample is butter, and the ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| recovery rate | aaaaa | aaaaa |
| recovery rate | aaaaa | aaaaa |
Login to View More
Abstract
The invention relates to a nondestructive test method for rapidly determining the content of aliphatic acid through applying front surface fluorescence. The method comprises the following steps: carrying out fluorescent scanning of a grease sample, collecting the obtained two-dimensional fluorescent spectral data, carrying out regression calculation of the fluorescent data and fatty acid data obtained through gas chromatography by using partial least square (PLS) to obtain a regression model; verifying to obtain that a predicted correlation coefficient of saturated aliphatic acid for the model, which is the correlation coefficient R<2> between the fluorescent spectrum and the aliphatic acid content, is 0.903-0.936 and the model has a very strong correlation; and using the established model to predict the content of the aliphatic acid in an unknown sample. Compared with routine detection methods, such as gas chromatography, the method provided by the invention has the advantages of simplicity, rapidness, nondestructive test, and substantial improvement of the detection efficiency through the utilization of the fluorescent technology, and can be practically used for detecting the content of the aliphatic acid.
Description
technical field [0001] The invention relates to a brand-new non-destructive testing method, in particular, the invention relates to a method which can directly and rapidly measure the fatty acid content in oil. Background technique [0002] The existing methods for determining the fatty acid composition of food are cumbersome and expensive. These limitations make these methods unable to be applied to the detection of large quantities of samples. For example, the most commonly used chromatographic method requires lengthy pretreatment steps, and fatty acids can be converted into fatty acid methyl esters (FAMEs) through saponification and esterification before they can be determined. [0003] In contrast, spectroscopic methods can integrate all the information on a single map, and usually do not require complicated pre-processing. Recently, there have been many reports on the application of spectroscopy in the fields of chemistry, pharmaceuticals, agricultural products, life s...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 周鹏刘小鸣彼得·恩塔卡萨内张燕萍梁丽
Owner JIANGNAN UNIV