Method for quickly measuring content of flavonoid constituents in paniculata

A technology for rapid determination of flavonoids, which is applied in measurement devices, material separation, analysis of materials, etc., can solve the problems of unpublished records, long time, low efficiency, etc., and achieves the effects of good stability, high sensitivity and simple determination method.

Active Publication Date: 2013-06-12
GUANGDONG SECOND TRADITIONAL CHINESE MEDICINE HOSPITAL (GUANGDONG PROVINCE ENG TECH RES INST OF TCM)
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Problems solved by technology

[0004] In the prior art, the HPLC method is used to only disclose the content determination of the four flavonoid components in Buza leaves, namely, epicatechin, vitexin, isovitexin and narcissin. The known rapid determination methods of HPLC are all One or two of the flavonoids are measured, and there is no public record of the rapid determination of the HPLC content of the four flavonoids at the same time. Therefore, the process is long, time-consuming, and inefficient.

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  • Method for quickly measuring content of flavonoid constituents in paniculata
  • Method for quickly measuring content of flavonoid constituents in paniculata
  • Method for quickly measuring content of flavonoid constituents in paniculata

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Embodiment Construction

[0024] Adopting HPLC method provided by the present invention, the steps of the rapid determination method of simultaneously measuring four kinds of flavonoid components of cloth dregs leaf are:

[0025] 1 Instruments and reagents

[0026] Agilent1200 high performance liquid chromatography, Mettler XS205DU electronic analytical balance (Switzerland).

[0027] The cloth dregs leaf samples were collected from various cities and counties in Guangdong Province and purchased from major pharmaceutical companies. A total of 10 samples were collected. All samples were identified by Professor Liu Fajin from the Guangdong Provincial Institute of Traditional Chinese Medicine as: Tiliaceae plant rag leaves Microcos paniculata L. The dried leaves are the varieties recorded in the 2010 edition of "Chinese Pharmacopoeia". Epicatechin (batch number: 878-200102), vitexin (batch number: 111687-200602) were purchased from China National Institute for the Control of Pharmaceutical and Biological...

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Abstract

The invention discloses a method for quickly measuring the content of flavonoid constituents in paniculata. The method is used for measuring the contents of epicatechin, vitexin, isovitexin and narcissoside in paniculata, wherein the contents can be quickly measured by using an HPLC (High Performance Liquid Chromatography) method. The method can be used for measuring the contents of the four flavonoid constituents, namely the epicatechin, the vitexin, the isovitexin and the narcissosid, in the paniculata simultaneously through one step. The measurement method provided by the invention has the advantages of high speed, simplicity, accuracy, high sensitivity and good stability, and is very beneficial to the application in quick quality control of medicinal materials of the paniculata in a drug preparation process.

Description

technical field [0001] The invention relates to a quality control method of traditional Chinese medicine, in particular to a rapid determination method for the content of flavonoids in Buza leaves, which can simultaneously measure four kinds of flavonoids in Buza leaves: epicatechin, vitexin, isovitexin and narciscoside The invention discloses a rapid determination of the content of the compound and belongs to the technical field of quality detection of traditional Chinese medicines. Background technique [0002] Cloth leaf is the dry leaf of Microcos paniculata L., a plant of Tiliaceae. It is produced in Guangdong, Guangxi, Hainan, Yunnan and other places. It is also distributed in India and Indonesia. In my country, Guangdong and Guangxi are rich in resources, of which Guangdong Yangxi and Zhanjiang are the main producing areas, and all of them are wild. This taste is slightly sour, cool in nature, non-toxic, has the effect of clearing heat and dampness, invigorating the s...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 孙冬梅罗文汇毕晓黎谭志灿胥爱丽李养学李素梅江洁怡
Owner GUANGDONG SECOND TRADITIONAL CHINESE MEDICINE HOSPITAL (GUANGDONG PROVINCE ENG TECH RES INST OF TCM)
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