Skin lightening composition
A composition, technology for the skin, applied in the field of creams for skin lightening kinetics
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Embodiment 1-6
[0047] Examples 1-6: Keratinocyte differentiation of disodium fumarate compared to other dicarboxylic acids
[0048] Several dicarboxylic acids were tested in in vitro assays (ie, keratinocyte differentiation), a well-known pathway leading to skin lightening. The test procedure is given below:
[0049] 5 x 104 HaCaT keratinocytes / well were grown in 24-well plates for 24 hours. The cells are incubated with the active substances for 24 hours. Cells were then washed with phosphate buffered saline (PBS) and stained with 5 g / ml acridine orange. Immediately observe cells using an epifluorescence microscope (Olympus BX 50) at 20X magnification using appropriate excitation and barrier filters while observing red and green fluorescence (excitation: 460 nm; emission >515 nm). Scan the entire plate to estimate the percentage of differentiated keratinocytes (cells with red fluorescence evenly distributed within the cytoplasm). Data are expressed as % reference. Increased numbers in...
Embodiment 7-10
[0054] Example 7-10: Melanin synthesis of disodium fumarate compared with other dicarboxylic acids
[0055] Put B16 melanocytes in 60mm 2 Incubate in a Petri dish for 2-3 days or until it reaches approximately 70% confluency, then add the active substance and incubate for 24 hours. Cells were harvested and protein content was estimated using the Bradford method. Cells were then lysed in 0.1N NaOH, and melanin was assessed. Data were then calculated as mg melanin / mg protein and expressed as % reference. A lower value indicates that the active substance inhibits melanin synthesis. The data are summarized in Table 3.
[0056] table 3
[0057] Example active substance concentration active substance average value # standard error t-test 7 15mM sebacic acid 87 3 0.04 8 10mM disodium fumarate 73 2 0.05 9 1 mM sodium butyrate 92 1 0.21 10 10mM sodium tartrate 69 2 0.04
[0058] # The data presented are the...
Embodiment 11 and 12
[0060] Examples 11 and 12: Skin Lightening Effect of Compositions with Disodium Fumarate and Selected Nonionic Surfactants
[0061] The compositions shown in Table 4 were prepared and tested for skin lightening using the protocol described below.
[0062] Rapid in vivo approach to short-term changes (MRIV)
[0063] This is a double-blind design in which neither the study subject nor the researcher applying the product is aware of the composition of the test product. Individuals with skin phototypes IV and V, skin color of 6.5-8.0 on the visual numerical scale (VNS) and no spots / scars / blemishes were selected for the study, where skin phototypes were determined according to Table 4 below:
[0064] Table 4
[0065] Types of originally also known as tanning properties Feature hair and eye color I Very shallow or "Celtic" Often burns, rarely tans freckles easily, red, brown, or blond hair, blue, brown, or gray eyes II Light or light-skinned ...
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