Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for detecting methylation degree for GCK (glucokinase) gene associated with type 2 diabetes and application thereof

A type 2 diabetes and methylation technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of non-disclosure, etc., and achieve the effect of strong pertinence and high detection efficiency.

Inactive Publication Date: 2013-06-19
NINGBO UNIV
View PDF1 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently no studies that demonstrate the relationship between gene methylation and type 2 diabetes
At the same time, there are no relevant research reports on the kits that can be used to detect the degree of methylation of the GCK gene associated with type 2 diabetes at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting methylation degree for GCK (glucokinase) gene associated with type 2 diabetes and application thereof
  • Kit for detecting methylation degree for GCK (glucokinase) gene associated with type 2 diabetes and application thereof
  • Kit for detecting methylation degree for GCK (glucokinase) gene associated with type 2 diabetes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment

[0021] 1. Collection of research objects

[0022] 48 patients with type 2 diabetes (24 males + 24 females) were collected from a hospital, and 48 age-sex-matched normal subjects were also collected as controls (24 males + 24 females).

[0023] 2. Genomic DNA extraction

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit for detecting methylation degree for GCK (glucokinase) gene associated with type 2 diabetes and application thereof. The kit is characterized by comprising a pair of GCK gene methylated specific amplification primers and methylated specific sequencing primers, wherein the upstream primer has the nucleotide sequence shown as SEQ ID NO.1 while the downstream primer has the nucleotide sequence shown as SEQ ID NO.2. The methylated specific sequencing primers are shown as SEQ ID NO.3. The kit has the advantages that the diagnostic kit can conveniently and quickly detect type 2 diabetes in molecular level with high detection efficiency and strong pertinence; and meanwhile, medicines taking GCK gene methylation as a target point are expected to become a novel means for auxiliary diagnosis, detection and screening of type 2 diabetes.

Description

technical field [0001] The invention relates to a detection kit for auxiliary diagnosis of type 2 diabetes, in particular to a detection kit for detecting the degree of methylation of the GCK gene associated with type 2 diabetes and its application Background technique [0002] Diabetes is an absolute or relative lack of insulin in the blood, leading to high blood sugar and diabetes, which in turn causes fat and protein metabolism disorders. Clinically, polyuria, polydipsia, polydipsia, polyphagia, weight loss and other manifestations may occur. Severe cases are prone to occur Acute complications such as ketoacidosis or chronic complications such as blood vessels and nerves. Diabetes is a common endocrine and metabolic disease, and its prevalence is gradually increasing with the improvement of living conditions and living standards. According to statistics, the number of diabetic patients in China is close to 100 million, and the prevalence rate is as high as 9.7%. Diabete...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 汤琳琳段世伟王钦文张莉娜徐雷艇步世忠
Owner NINGBO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com