Kit for identifying and detecting sheep pox viruses and goat pox viruses
A technology of sheep pox virus and goat pox virus, applied in the direction of microbial determination/testing, biochemical equipment and methods, etc., can solve the problem of inability to distinguish sheep pox virus and goat pox virus, low detection sensitivity, and inability to distinguish Problems such as viruses of the same genus and different species
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[0029] The present invention is explained in detail below in conjunction with embodiment.
[0030] 1. Preparation of Sequences
[0031]Use the online lamp primer design software (PrimerExplorerV3) to design three sets of primers for the inverted repeat end (within 1-2000bp) of the sheeppox virus genome, and select the primers with higher scores from the hundreds of pairs of primers obtained. Sequences were sent to Nanjing KingScript Bioengineering Co., Ltd. for 5' end modification synthesis.
[0032] 2. Viral Genome Extraction
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