Kit for identifying and detecting sheep pox viruses and goat pox viruses

Abstract

The invention discloses a kit for identifying and detecting sheep pox viruses and goat pox viruses. The kit comprises three groups of primers: SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11 and SEQ ID NO. 12. The kit can quickly identify the sheep pox viruses and the goat pox viruses and can be applied to epidemiologic research on sheep and goat pot viruses.

Description

technical field [0001] The invention relates to a detection kit, specifically a kit for detecting sheep pox virus and goat pox virus. Background technique [0002] Capripox (CP) includes sheep pox, goat pox and bovine skin pimple disease, the first two of which are caused by sheep pox virus (Sheeppox virus, SPPV) and goat pox virus (Goatpox virus, GTPV) respectively. contact infectious diseases. The body temperature of sick animals rises, papules or nodules, blisters, visceral lesions and even death appear on the whole body, which has caused serious economic losses to the sheep industry in the world. The World Organization for Animal Health (OIE) stipulates it as an animal disease that must be reported by law, and my country lists it as a first-class animal infectious disease. The differential diagnosis of sheep pox and goat pox has been a research hotspot. [0003] Studies have shown that the homology of SPPV and GTPV gene sequences reaches 96%-97%, and there are only c...

AI Technical Summary

Problems solved by technology

The study targeted the conserved VP39 gene shared by the three pathogens of SPPV, GTPV and LSDV, so this method cannot distinguish between sheep pox virus and goat pox virus; while Li Yingguo and others from the Chongqing Entry-Exit Inspection and Quarantine Bureau in China also established a detection For the Lamp diagnostic kit of the genus Goatpox, refer to: Primers, kits and detection methods for rapid detection of Goatpox virus isothermal amplification technology, patent number: CN102373302A, this method is also shared by the three pathogens of SPPV, GTPV and LSDV The 1119272bp-120322bp conserved sequence of the genome is the target gene

Therefore, the methods or kits established by them can only detect the virus of the genus of goat poxvirus, but cannot distinguish between viruses of the same genus and different species, that is, they cannot distinguish between the sheep pox virus and the goat pox virus.

At the same time, these methods also have the disadvantages of low detection sensitivity, which is unfavorable for virus detection.

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